The possibility of using fibrin-based collagen as an antibiotic delivery system

Junichi Nishimura, Kiyokazu Nakajima, Yoshihito Souma, Tsuyoshi Takahashi, Naoki Ikeguchi, Rei Takenaka, Naoki Shinohara, Toshirou Nishida, Yuichiro Doki, Masaki Mori

Research output: Contribution to journalArticle

Abstract

Purpose: Collagen and fibrin are known to have potential use as a local drug-delivery system. This experimental study was designed to evaluate whether a fibrinogen-based collagen (FBC) fleece, coated with thrombin and aprotinin, can be used as an antibiotic delivery system. Methods: In an in vitro study, gentamicin, fosfomycin, ampicillin, ciprofloxacin and dibekacin were absorbed by the FBC, Kirby-Bauer disks (KBDs), and expanded polytetrafluoroethylene. After washing with saline or phosphate buffer saline (PBS) 3 times for 6, 12 and 24 h, each sample was analyzed for antibiotic retention. In an in vivo study, we implanted the FBC onto mouse livers and dripped gentamicin and ciprofloxacin onto the FBC. The FBCs were subsequently collected and analyzed for their antibiotic activities. Results: After irrigation with saline, each antibiotic showed different activities. After PBS washing, the FBC impregnated with each antibiotic had higher activity than the KBDs, and inhibited the bacterial growth by 60-80 % compared to the control. Gentamicin dripped onto the FBC could inhibit bacterial growth after 48 h in vivo without affecting the hemostatic properties of the FBC. However, the FBC treated with ciprofloxacin exhibited antibacterial activity for only 3 h. Conclusions: Some bases, including FBC, can retain antibacterial activities dependent on the ingredients of the base and the type of antibiotic. Gentamicin, but not ciprofloxacin, was retained in the FBC in vivo. These results suggest that absorbent FBC might be useful not only as hemostatic material, but also as a local drug-delivery system.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalSurgery today
Volume43
Issue number2
DOIs
Publication statusPublished - Feb 1 2013
Externally publishedYes

Fingerprint

Fibrin
Fibrinogen
Collagen
Anti-Bacterial Agents
Ciprofloxacin
Gentamicins
Hemostatics
Drug Delivery Systems
Buffers
Dibekacin
Phosphates
Fosfomycin
Aprotinin
Polytetrafluoroethylene
Ampicillin
Growth
Thrombin

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Nishimura, J., Nakajima, K., Souma, Y., Takahashi, T., Ikeguchi, N., Takenaka, R., ... Mori, M. (2013). The possibility of using fibrin-based collagen as an antibiotic delivery system. Surgery today, 43(2), 185-190. https://doi.org/10.1007/s00595-012-0210-0

The possibility of using fibrin-based collagen as an antibiotic delivery system. / Nishimura, Junichi; Nakajima, Kiyokazu; Souma, Yoshihito; Takahashi, Tsuyoshi; Ikeguchi, Naoki; Takenaka, Rei; Shinohara, Naoki; Nishida, Toshirou; Doki, Yuichiro; Mori, Masaki.

In: Surgery today, Vol. 43, No. 2, 01.02.2013, p. 185-190.

Research output: Contribution to journalArticle

Nishimura, J, Nakajima, K, Souma, Y, Takahashi, T, Ikeguchi, N, Takenaka, R, Shinohara, N, Nishida, T, Doki, Y & Mori, M 2013, 'The possibility of using fibrin-based collagen as an antibiotic delivery system', Surgery today, vol. 43, no. 2, pp. 185-190. https://doi.org/10.1007/s00595-012-0210-0
Nishimura J, Nakajima K, Souma Y, Takahashi T, Ikeguchi N, Takenaka R et al. The possibility of using fibrin-based collagen as an antibiotic delivery system. Surgery today. 2013 Feb 1;43(2):185-190. https://doi.org/10.1007/s00595-012-0210-0
Nishimura, Junichi ; Nakajima, Kiyokazu ; Souma, Yoshihito ; Takahashi, Tsuyoshi ; Ikeguchi, Naoki ; Takenaka, Rei ; Shinohara, Naoki ; Nishida, Toshirou ; Doki, Yuichiro ; Mori, Masaki. / The possibility of using fibrin-based collagen as an antibiotic delivery system. In: Surgery today. 2013 ; Vol. 43, No. 2. pp. 185-190.
@article{bdd4d759e60142c9805ecb1e025c91e4,
title = "The possibility of using fibrin-based collagen as an antibiotic delivery system",
abstract = "Purpose: Collagen and fibrin are known to have potential use as a local drug-delivery system. This experimental study was designed to evaluate whether a fibrinogen-based collagen (FBC) fleece, coated with thrombin and aprotinin, can be used as an antibiotic delivery system. Methods: In an in vitro study, gentamicin, fosfomycin, ampicillin, ciprofloxacin and dibekacin were absorbed by the FBC, Kirby-Bauer disks (KBDs), and expanded polytetrafluoroethylene. After washing with saline or phosphate buffer saline (PBS) 3 times for 6, 12 and 24 h, each sample was analyzed for antibiotic retention. In an in vivo study, we implanted the FBC onto mouse livers and dripped gentamicin and ciprofloxacin onto the FBC. The FBCs were subsequently collected and analyzed for their antibiotic activities. Results: After irrigation with saline, each antibiotic showed different activities. After PBS washing, the FBC impregnated with each antibiotic had higher activity than the KBDs, and inhibited the bacterial growth by 60-80 {\%} compared to the control. Gentamicin dripped onto the FBC could inhibit bacterial growth after 48 h in vivo without affecting the hemostatic properties of the FBC. However, the FBC treated with ciprofloxacin exhibited antibacterial activity for only 3 h. Conclusions: Some bases, including FBC, can retain antibacterial activities dependent on the ingredients of the base and the type of antibiotic. Gentamicin, but not ciprofloxacin, was retained in the FBC in vivo. These results suggest that absorbent FBC might be useful not only as hemostatic material, but also as a local drug-delivery system.",
author = "Junichi Nishimura and Kiyokazu Nakajima and Yoshihito Souma and Tsuyoshi Takahashi and Naoki Ikeguchi and Rei Takenaka and Naoki Shinohara and Toshirou Nishida and Yuichiro Doki and Masaki Mori",
year = "2013",
month = "2",
day = "1",
doi = "10.1007/s00595-012-0210-0",
language = "English",
volume = "43",
pages = "185--190",
journal = "Surgery Today",
issn = "0941-1291",
publisher = "Springer Japan",
number = "2",

}

TY - JOUR

T1 - The possibility of using fibrin-based collagen as an antibiotic delivery system

AU - Nishimura, Junichi

AU - Nakajima, Kiyokazu

AU - Souma, Yoshihito

AU - Takahashi, Tsuyoshi

AU - Ikeguchi, Naoki

AU - Takenaka, Rei

AU - Shinohara, Naoki

AU - Nishida, Toshirou

AU - Doki, Yuichiro

AU - Mori, Masaki

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Purpose: Collagen and fibrin are known to have potential use as a local drug-delivery system. This experimental study was designed to evaluate whether a fibrinogen-based collagen (FBC) fleece, coated with thrombin and aprotinin, can be used as an antibiotic delivery system. Methods: In an in vitro study, gentamicin, fosfomycin, ampicillin, ciprofloxacin and dibekacin were absorbed by the FBC, Kirby-Bauer disks (KBDs), and expanded polytetrafluoroethylene. After washing with saline or phosphate buffer saline (PBS) 3 times for 6, 12 and 24 h, each sample was analyzed for antibiotic retention. In an in vivo study, we implanted the FBC onto mouse livers and dripped gentamicin and ciprofloxacin onto the FBC. The FBCs were subsequently collected and analyzed for their antibiotic activities. Results: After irrigation with saline, each antibiotic showed different activities. After PBS washing, the FBC impregnated with each antibiotic had higher activity than the KBDs, and inhibited the bacterial growth by 60-80 % compared to the control. Gentamicin dripped onto the FBC could inhibit bacterial growth after 48 h in vivo without affecting the hemostatic properties of the FBC. However, the FBC treated with ciprofloxacin exhibited antibacterial activity for only 3 h. Conclusions: Some bases, including FBC, can retain antibacterial activities dependent on the ingredients of the base and the type of antibiotic. Gentamicin, but not ciprofloxacin, was retained in the FBC in vivo. These results suggest that absorbent FBC might be useful not only as hemostatic material, but also as a local drug-delivery system.

AB - Purpose: Collagen and fibrin are known to have potential use as a local drug-delivery system. This experimental study was designed to evaluate whether a fibrinogen-based collagen (FBC) fleece, coated with thrombin and aprotinin, can be used as an antibiotic delivery system. Methods: In an in vitro study, gentamicin, fosfomycin, ampicillin, ciprofloxacin and dibekacin were absorbed by the FBC, Kirby-Bauer disks (KBDs), and expanded polytetrafluoroethylene. After washing with saline or phosphate buffer saline (PBS) 3 times for 6, 12 and 24 h, each sample was analyzed for antibiotic retention. In an in vivo study, we implanted the FBC onto mouse livers and dripped gentamicin and ciprofloxacin onto the FBC. The FBCs were subsequently collected and analyzed for their antibiotic activities. Results: After irrigation with saline, each antibiotic showed different activities. After PBS washing, the FBC impregnated with each antibiotic had higher activity than the KBDs, and inhibited the bacterial growth by 60-80 % compared to the control. Gentamicin dripped onto the FBC could inhibit bacterial growth after 48 h in vivo without affecting the hemostatic properties of the FBC. However, the FBC treated with ciprofloxacin exhibited antibacterial activity for only 3 h. Conclusions: Some bases, including FBC, can retain antibacterial activities dependent on the ingredients of the base and the type of antibiotic. Gentamicin, but not ciprofloxacin, was retained in the FBC in vivo. These results suggest that absorbent FBC might be useful not only as hemostatic material, but also as a local drug-delivery system.

UR - http://www.scopus.com/inward/record.url?scp=84878504149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878504149&partnerID=8YFLogxK

U2 - 10.1007/s00595-012-0210-0

DO - 10.1007/s00595-012-0210-0

M3 - Article

VL - 43

SP - 185

EP - 190

JO - Surgery Today

JF - Surgery Today

SN - 0941-1291

IS - 2

ER -