The possible role of nitric oxide in relaxations and excitatory neuroeffector transmission in the cat airway

Hiroyuki Tanaka, Liang Jing, Shosuke Takahashi, Yushi Ito

Research output: Contribution to journalArticle

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Abstract

1. To study the possible role of nitric oxide (NO free radical; NO) or NO-containing compounds in the non-adrenergic, non-cholinergic (NANC) relaxations, we observed the effects of carboxy-2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (C-PTIO; a newly synthesized NO scavenger) on NANC relaxations in the cat airway. In addition, we also observed the effects of C-PTIO on excitatory junction potentials (EJPs), since NO· has a prejunctional action on transmitter release. 2. Nitrosocystine (Cys-NO) (10 -7 -10 -3 M) dose-dependently relaxed the bronchial tissue in the presence of 5-HT, atropine and guanethidine and C-PTIO (10 -4 M) Shifted the concentration-response curve of the Cys-NO to the right. 3. Electrical field stimulation (EFS) evoked biphasic NANC relaxations in the small bronchi of the cat. In general, C-PTIO suppressed non-selectively both the first and second components of the NANC relaxations to a similar extent. However, in some bronchial preparations C-PTIO (10 -4 M) selectively suppressed the first component of the NANC relaxation to approximately 50% of the initial value, enhancing the amplitude of the second component of the NANC relaxations. 4. After pretreatment of the bronchial tissues with α-chymotrypsin (1 unit ml -1 ) for 30 min in order to inhibit any response to peptides, EFS evoked monophasic NANC relaxation. C-PTIO (10 -5 -10 -4 M) dose-dependently suppressed, and at a concentration of 10 -4 M almost halved, the amplitude of NANC relaxation. Additional application of L-NAME further reduced the C-PTIO-resistant NANC relaxation to 20-30% of the initial value. 5. C-PTIO (10 -4 M) enhanced the EJP amplitude evoked by single EFS in the trachea but not in the bronchi. However, C-PTIO enhanced the summation of the EJPs to repeated stimulation to a similar extent in the tracheal and bronchial tissues. Simultaneous application of C-PTIO and L-NAME did not further enhance the summation. 6. These results indicate that NO and NO-containing compounds are involved in the L-NAME-sensitive NANC relaxation in the cat airway, and that only NO. has a prejunctional action which inhibits excitatory neuroeffector transmission.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalJournal of Physiology
Volume493
Issue number3
DOIs
Publication statusPublished - Jun 15 1996

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Nitric Oxide
Cats
NG-Nitroarginine Methyl Ester
Electric Stimulation
Bronchi
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
Imidazolines
Guanethidine
Chymotrypsin
Trachea
Atropine
Evoked Potentials
Oxides
Free Radicals
Serotonin
Peptides

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

The possible role of nitric oxide in relaxations and excitatory neuroeffector transmission in the cat airway. / Tanaka, Hiroyuki; Jing, Liang; Takahashi, Shosuke; Ito, Yushi.

In: Journal of Physiology, Vol. 493, No. 3, 15.06.1996, p. 785-791.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Jing, Liang ; Takahashi, Shosuke ; Ito, Yushi. / The possible role of nitric oxide in relaxations and excitatory neuroeffector transmission in the cat airway. In: Journal of Physiology. 1996 ; Vol. 493, No. 3. pp. 785-791.
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