The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs

Daijiro Konno, Ji Ae Ko, Shinichi Usui, Kei Hori, Hisato Maruoka, Makoto Inui, Takashi Fujikado, Yasuo Tano, Tatsuo Suzuki, Koujiro Tohyama, Kenji Sobue

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

The postsynaptic site of the excitatory synapse, which is composed of the postsynaptic density (PSD) attached to the postsynaptic membrane, is a center for synaptic plasticity. To reveal the molecular organization and functional regulation of the postsynaptic site, we cloned a 70 kDa protein that is concentrated in PSDs using a monoclonal antibody against the PSD. This protein, named PSD-Zip70, is highly homologous to the human FEZ1/LZTS1 gene product. PSD-Zip70 contains an N-myristoylation consensus sequence, a polybasic cluster in the N-terminal region and four leucine-zipper motifs in the C-terminal region. Light and electron microscopy showed that this protein was localized to the dendritic spines, especially in the PSD and the postsynaptic membrane. Fractionation of the synaptic plasma membrane demonstrated that PSD-Zip70 was localized to the PSD and the dendritic raft. In Madin-Darby canine kidney (NMCK) cells, exogenous PSD-Zip70 was targeted to the apical plasma membrane of microvilli, and its N-myristoylation was necessary for this targeting. In hippocampal neurons, N-myristoylation was also required for the membrane localization and the C-terminal region was critically involved in the synaptic targeting. These results suggest that PSD-Zip70 may be involved in the dynamic properties of the structure and function of the postsynaptic site.

Original languageEnglish
Pages (from-to)4695-4706
Number of pages12
JournalJournal of Cell Science
Volume115
Issue number23
DOIs
Publication statusPublished - Dec 1 2002

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Post-Synaptic Density
Leucine Zippers
Membranes
Cell Membrane
Synaptic Membranes
Dendritic Spines
Madin Darby Canine Kidney Cells
Neuronal Plasticity
Consensus Sequence
Microvilli
Synapses
Electron Microscopy
Proteins
Monoclonal Antibodies
Neurons
Light

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs. / Konno, Daijiro; Ko, Ji Ae; Usui, Shinichi; Hori, Kei; Maruoka, Hisato; Inui, Makoto; Fujikado, Takashi; Tano, Yasuo; Suzuki, Tatsuo; Tohyama, Koujiro; Sobue, Kenji.

In: Journal of Cell Science, Vol. 115, No. 23, 01.12.2002, p. 4695-4706.

Research output: Contribution to journalReview article

Konno, D, Ko, JA, Usui, S, Hori, K, Maruoka, H, Inui, M, Fujikado, T, Tano, Y, Suzuki, T, Tohyama, K & Sobue, K 2002, 'The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs', Journal of Cell Science, vol. 115, no. 23, pp. 4695-4706. https://doi.org/10.1242/jcs.00127
Konno, Daijiro ; Ko, Ji Ae ; Usui, Shinichi ; Hori, Kei ; Maruoka, Hisato ; Inui, Makoto ; Fujikado, Takashi ; Tano, Yasuo ; Suzuki, Tatsuo ; Tohyama, Koujiro ; Sobue, Kenji. / The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs. In: Journal of Cell Science. 2002 ; Vol. 115, No. 23. pp. 4695-4706.
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