The PPARγ locus makes long-range chromatin interactions with selected tissue-specific gene loci during adipocyte differentiation in a protein kinase a dependent manner

Scott E. LeBlanc, Qiong Wu, A. Rasim Barutcu, Hengyi Xiao, Yasuyuki Ohkawa, Anthony N. Imbalzano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Differentiation signaling results in reprogramming of cellular gene expression that leads to morphological changes and functional specialization of a precursor cell. This global change in gene expression involves temporal regulation of differentiation-specific genes that are located throughout the genome, raising the idea that genome structure may also be re-organized during cell differentiation to facilitate regulated gene expression. Using in vitro adipocyte differentiation as a model, we explored whether gene organization within the nucleus is altered upon exposure of precursor cells to signaling molecules that induce adipogenesis. The peroxisome proliferator-activated receptor gamma (PPARγ) nuclear hormone receptor is a master determinant of adipogenesis and is required for adipose differentiation. We utilized the chromosome conformation capture (3C) assay to determine whether the position of the PPARc locus relative to other adipogenic genes is changed during differentiation. We report that the PPARγ2 promoter is transiently positioned in proximity to the promoters of genes encoding adipokines and lipid droplet associated proteins at 6 hours post-differentiation, a time that precedes expression of any of these genes. In contrast, the PPARγ2 promoter was not in proximity to the EF1α promoter, which drives expression of a constitutively active, housekeeping gene that encodes a translation elongation factor, nor was the PPARγ2 promoter in proximity to the promoter driving the expression of the C/EBPα regulatory protein. The formation of the long-range, intergenic interactions involving the PPARγ2 promoter required the regulatory factor C/EBPβ, elevated cyclic AMP (cAMP) levels, and protein kinase A (PKA) signaling. We conclude that genome organization is dynamically remodeled in response to adipogenic signaling, and we speculate that these transient inter-genic interactions may be formed for the purposes of selecting some of the transcriptionally silent tissue-specific loci for subsequent transcriptional activation.

Original languageEnglish
Article numbere86140
JournalPloS one
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 20 2014

Fingerprint

PPAR gamma
adipocytes
Adipocytes
protein kinases
Protein Kinases
Chromatin
chromatin
Genes
promoter regions
Tissue
Gene Expression
Adipogenesis
loci
Genome
genes
Gene expression
CCAAT-Enhancer-Binding Proteins
Peptide Elongation Factors
Adenylate Kinase
Adipokines

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

The PPARγ locus makes long-range chromatin interactions with selected tissue-specific gene loci during adipocyte differentiation in a protein kinase a dependent manner. / LeBlanc, Scott E.; Wu, Qiong; Barutcu, A. Rasim; Xiao, Hengyi; Ohkawa, Yasuyuki; Imbalzano, Anthony N.

In: PloS one, Vol. 9, No. 1, e86140, 20.01.2014.

Research output: Contribution to journalArticle

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