Abstract
Background: Jumonji-domain containing 3 (JMJD3) affects transcriptional regulation by demethylating lysine 27 residue of histone 3. We investigated its function and prognostic significance in colorectal cancer (CRC). Methods: The influence of JMJD3 on cell proliferation was assessed using quantitative RT-PCR and western blot on the downstream target gene of JMJD3, in knock-down (KD) experiments and clinical samples from 151 CRC patients. Results: Cells with KD JMJD3 significantly increased proliferation through cell cycle progression and apoptosis suppression. Expression of P15INK4B was remarkably decreased in KD JMJD3 cells; and JMJD3 expression strongly correlated with p15INK4B expression in clinical CRC samples (P < 0.001, r = 0.566). Low JMJD3 also was an independent predictor of poor prognosis (P = 0.042) in surgically resected CRC patients. Conclusions: JMJD3 has prognostic significance in CRC and mediates p15INK4B expression. These results imply that elucidation of the JMJD3 role may lead to a new therapeutic approach for CRC patients.
| Original language | English |
|---|---|
| Pages (from-to) | 678-685 |
| Number of pages | 8 |
| Journal | Annals of Surgical Oncology |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2016 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Oncology
Cite this
The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in Colorectal Cancer. / Tokunaga, Ryuma; Sakamoto, Yasuo; Nakagawa, Shigeki; Miyake, Keisuke; Izumi, Daisuke; Kosumi, Keisuke; Taki, Katsunobu; Higashi, Takaaki; Imamura, Yu; Ishimoto, Takatsugu; Iwatsuki, Masaaki; Baba, Yoshifumi; Miyamoto, Yuji; Yoshida, Naoya; Oki, Eiji; Watanabe, Masayuki; Baba, Hideo.
In: Annals of Surgical Oncology, Vol. 23, No. 2, 01.02.2016, p. 678-685.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in Colorectal Cancer
AU - Tokunaga, Ryuma
AU - Sakamoto, Yasuo
AU - Nakagawa, Shigeki
AU - Miyake, Keisuke
AU - Izumi, Daisuke
AU - Kosumi, Keisuke
AU - Taki, Katsunobu
AU - Higashi, Takaaki
AU - Imamura, Yu
AU - Ishimoto, Takatsugu
AU - Iwatsuki, Masaaki
AU - Baba, Yoshifumi
AU - Miyamoto, Yuji
AU - Yoshida, Naoya
AU - Oki, Eiji
AU - Watanabe, Masayuki
AU - Baba, Hideo
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Background: Jumonji-domain containing 3 (JMJD3) affects transcriptional regulation by demethylating lysine 27 residue of histone 3. We investigated its function and prognostic significance in colorectal cancer (CRC). Methods: The influence of JMJD3 on cell proliferation was assessed using quantitative RT-PCR and western blot on the downstream target gene of JMJD3, in knock-down (KD) experiments and clinical samples from 151 CRC patients. Results: Cells with KD JMJD3 significantly increased proliferation through cell cycle progression and apoptosis suppression. Expression of P15INK4B was remarkably decreased in KD JMJD3 cells; and JMJD3 expression strongly correlated with p15INK4B expression in clinical CRC samples (P < 0.001, r = 0.566). Low JMJD3 also was an independent predictor of poor prognosis (P = 0.042) in surgically resected CRC patients. Conclusions: JMJD3 has prognostic significance in CRC and mediates p15INK4B expression. These results imply that elucidation of the JMJD3 role may lead to a new therapeutic approach for CRC patients.
AB - Background: Jumonji-domain containing 3 (JMJD3) affects transcriptional regulation by demethylating lysine 27 residue of histone 3. We investigated its function and prognostic significance in colorectal cancer (CRC). Methods: The influence of JMJD3 on cell proliferation was assessed using quantitative RT-PCR and western blot on the downstream target gene of JMJD3, in knock-down (KD) experiments and clinical samples from 151 CRC patients. Results: Cells with KD JMJD3 significantly increased proliferation through cell cycle progression and apoptosis suppression. Expression of P15INK4B was remarkably decreased in KD JMJD3 cells; and JMJD3 expression strongly correlated with p15INK4B expression in clinical CRC samples (P < 0.001, r = 0.566). Low JMJD3 also was an independent predictor of poor prognosis (P = 0.042) in surgically resected CRC patients. Conclusions: JMJD3 has prognostic significance in CRC and mediates p15INK4B expression. These results imply that elucidation of the JMJD3 role may lead to a new therapeutic approach for CRC patients.
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UR - http://www.scopus.com/inward/citedby.url?scp=84958165934&partnerID=8YFLogxK
U2 - 10.1245/s10434-015-4879-3
DO - 10.1245/s10434-015-4879-3
M3 - Article
C2 - 26416711
AN - SCOPUS:84958165934
VL - 23
SP - 678
EP - 685
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
SN - 1068-9265
IS - 2
ER -