The promise of targeted proteomics for quantitative network biology

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Proteomics is a powerful tool for obtaining information on a large number of proteins with regard to their expression levels, interactions with other molecules, and posttranslational modifications. Whereas nontargeted, discovery proteomics uncovers differences in the proteomic landscape under different conditions, targeted proteomics has been developed to overcome the limitations of this approach with regard to quantitation. In addition to technical advances in instruments and informatics tools, the advent of the synthetic proteome composed of synthetic peptides or recombinant proteins has advanced the adoption of targeted proteomics across a wide range of research fields. Targeted proteomics can now be applied to measurement of the dynamics of any proteins of interest under a variety of conditions as well as to estimation of the absolute abundance or stoichiometry of proteins in a given network. Multiplexed targeted proteomics assays of high reproducibility and accuracy can provide insight at the quantitative level into entire networks that govern biological phenomena or diseases. Such assays will establish a new paradigm for data-driven science.

Original languageEnglish
Pages (from-to)88-97
Number of pages10
JournalCurrent Opinion in Biotechnology
Volume54
DOIs
Publication statusPublished - Dec 1 2018

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Proteomics
Proteins
Assays
Biological Phenomena
Recombinant proteins
Informatics
Proteome
Post Translational Protein Processing
Recombinant Proteins
Stoichiometry
Peptides
Molecules
Research

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The promise of targeted proteomics for quantitative network biology. / Matsumoto, Masaki; Nakayama, Keiichi.

In: Current Opinion in Biotechnology, Vol. 54, 01.12.2018, p. 88-97.

Research output: Contribution to journalReview article

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