Abstract
Both gamma rays and atmospheric pressure plasma are known to have anticancer properties. While their mechanism actions are still not clear, in some contexts they work in similar manner, while in other contexts they work differently. So to understand these relationships, we have studied Myoglobin protein after the treatment of gamma rays and dielectric barrier discharge (DBD) plasma, and analyzed the changes in thermodynamic properties and changes in the secondary structure of protein after both treatments. The thermodynamic properties were analyzed using chemical and thermal denaturation after both treatments. We have also studied the action of gamma rays and DBD plasma on myoglobin in the presence of osmolytes, such as sorbitol and trehalose. For deep understanding of the action of gamma rays and DBD plasma, we have analyzed the reactive species generated by them in buffer at all treatment conditions. Finally, we have used molecular dynamic simulation to understand the hydrogen peroxide action on myoglobin with or without osmolytes, to gain deeper insight into how the osmolytes can protect the protein structure from the reactive species generated by gamma rays and DBD plasma.
| Original language | English |
|---|---|
| Article number | 8698 |
| Journal | Scientific reports |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 1 2017 |
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All Science Journal Classification (ASJC) codes
- General
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The protective action of osmolytes on the deleterious effects of gamma rays and atmospheric pressure plasma on protein conformational changes. / Attri, Pankaj; Kim, Minsup; Sarinont, Thapanut; Choi, Eun Ha; Seo, Hyunwoong; Cho, Art E.; Koga, Kazunori; Shiratani, Masaharu.
In: Scientific reports, Vol. 7, No. 1, 8698, 01.12.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The protective action of osmolytes on the deleterious effects of gamma rays and atmospheric pressure plasma on protein conformational changes
AU - Attri, Pankaj
AU - Kim, Minsup
AU - Sarinont, Thapanut
AU - Choi, Eun Ha
AU - Seo, Hyunwoong
AU - Cho, Art E.
AU - Koga, Kazunori
AU - Shiratani, Masaharu
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Both gamma rays and atmospheric pressure plasma are known to have anticancer properties. While their mechanism actions are still not clear, in some contexts they work in similar manner, while in other contexts they work differently. So to understand these relationships, we have studied Myoglobin protein after the treatment of gamma rays and dielectric barrier discharge (DBD) plasma, and analyzed the changes in thermodynamic properties and changes in the secondary structure of protein after both treatments. The thermodynamic properties were analyzed using chemical and thermal denaturation after both treatments. We have also studied the action of gamma rays and DBD plasma on myoglobin in the presence of osmolytes, such as sorbitol and trehalose. For deep understanding of the action of gamma rays and DBD plasma, we have analyzed the reactive species generated by them in buffer at all treatment conditions. Finally, we have used molecular dynamic simulation to understand the hydrogen peroxide action on myoglobin with or without osmolytes, to gain deeper insight into how the osmolytes can protect the protein structure from the reactive species generated by gamma rays and DBD plasma.
AB - Both gamma rays and atmospheric pressure plasma are known to have anticancer properties. While their mechanism actions are still not clear, in some contexts they work in similar manner, while in other contexts they work differently. So to understand these relationships, we have studied Myoglobin protein after the treatment of gamma rays and dielectric barrier discharge (DBD) plasma, and analyzed the changes in thermodynamic properties and changes in the secondary structure of protein after both treatments. The thermodynamic properties were analyzed using chemical and thermal denaturation after both treatments. We have also studied the action of gamma rays and DBD plasma on myoglobin in the presence of osmolytes, such as sorbitol and trehalose. For deep understanding of the action of gamma rays and DBD plasma, we have analyzed the reactive species generated by them in buffer at all treatment conditions. Finally, we have used molecular dynamic simulation to understand the hydrogen peroxide action on myoglobin with or without osmolytes, to gain deeper insight into how the osmolytes can protect the protein structure from the reactive species generated by gamma rays and DBD plasma.
UR - http://www.scopus.com/inward/record.url?scp=85027854890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027854890&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-08643-1
DO - 10.1038/s41598-017-08643-1
M3 - Article
C2 - 28821765
AN - SCOPUS:85027854890
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 8698
ER -