The protein arginine methyltransferase Prmt5 is required for myogenesis because it facilitates ATP-dependent chromatin remodeling

Caroline S. Dacwag, Yasuyuki Ohkawa, Sharmistha Pal, Saíd Sif, Anthony N. Imbalzano

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Skeletal muscle differentiation requires the coordinated activity of transcription factors, histone modifying enzymes, and ATP-dependent chromatin remodeling enzymes. The type II protein arginine methyltransferase Prmt5 symmetrically dimethylates histones H3 and H4 and numerous nonchromatin proteins, and prior work has implicated Prmt5 in transcriptional repression. Here we demonstrate that MyoD-induced muscle differentiation requires Prmt5. One of the first genes activated during differentiation encodes the myogenic regulator myogenin. Prmt5 and dimethylated H3R8 (histone 3 arginine 8) are localized at the myogenin promoter in differentiating cells. Modification of H3R8 required Prmt5, and reduction of Prmt5 resulted in the abrogation of promoter binding by the Brg1 ATPase-associated with the SWI/SNF chromatin remodeling enzymes and all subsequent events associated with gene activation, including increases in chromatin accessibility and stable binding by MyoD. Prmt5 and dimethylated H3R8 were also associated with the myogenin promoter in activated satellite cells isolated from muscle tissue, further demonstrating the physiological relevance of these observations. The data indicate that Prmt5 facilitates myogenesis because it is required for Brg1-dependent chromatin remodeling and gene activation at a locus essential for differentiation. We therefore conclude that a histone modifying enzyme is necessary to permit an ATP-dependent chromatin remodeling enzyme to function.

Original languageEnglish
Pages (from-to)384-394
Number of pages11
JournalMolecular and cellular biology
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

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Protein-Arginine N-Methyltransferases
Chromatin Assembly and Disassembly
Muscle Development
Histones
Adenosine Triphosphate
Myogenin
Enzymes
Arginine
Transcriptional Activation
Histone Code
Muscles
Chromatin
Adenosine Triphosphatases
Skeletal Muscle
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

The protein arginine methyltransferase Prmt5 is required for myogenesis because it facilitates ATP-dependent chromatin remodeling. / Dacwag, Caroline S.; Ohkawa, Yasuyuki; Pal, Sharmistha; Sif, Saíd; Imbalzano, Anthony N.

In: Molecular and cellular biology, Vol. 27, No. 1, 01.01.2007, p. 384-394.

Research output: Contribution to journalArticle

Dacwag, Caroline S. ; Ohkawa, Yasuyuki ; Pal, Sharmistha ; Sif, Saíd ; Imbalzano, Anthony N. / The protein arginine methyltransferase Prmt5 is required for myogenesis because it facilitates ATP-dependent chromatin remodeling. In: Molecular and cellular biology. 2007 ; Vol. 27, No. 1. pp. 384-394.
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