The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans

Taiki Futagami, Kazufumi Seto, Yasuhiro Kajiwara, Hideharu Takashita, Toshiro Omori, Kaoru Takegawa, Masatoshi Goto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The Mid2-like protein MtlA is a putative sensor of the cell wall integrity (CWI) signaling pathway in Aspergillus nidulans. An MtlA-EGFP fusion protein was localized at the cell surface and septa. The mtlA disruptant (ΔmtlA) showed radial colony growth similar to the wild-type (wt) strain, but showed reduced conidia formation. The ΔmtlA mutant showed growth deficiency in the presence of inhibitors of cell wall synthesis. Moreover, mtlA disruption resulted in a reduction in the glucan and chitin content in the cell wall. These results suggest that MtlA plays a significant role in asexual sporulation, cell wall stress tolerance, and the maintenance of CWI in A. nidulans, but transcriptional upregulation of α-1,3-glucan synthase gene agsB induced by micafungin was observed in the ΔmtlA strain as well as the wt strain. Thus, MtlA is not essential for activation of the downstream CWI signaling pathway components identified in previous studies of Saccharomyces cerevisiae.

Original languageEnglish
Pages (from-to)326-335
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number2
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Aspergillus nidulans
Fungal Spores
Aspergillus
Heat-Shock Proteins
Cell Wall
Cells
Sensors
Proteins
Chitin
Glucans
Yeast
Fusion reactions
Genes
Chemical activation
Growth
Saccharomyces cerevisiae
Up-Regulation
Maintenance

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans. / Futagami, Taiki; Seto, Kazufumi; Kajiwara, Yasuhiro; Takashita, Hideharu; Omori, Toshiro; Takegawa, Kaoru; Goto, Masatoshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 2, 01.01.2014, p. 326-335.

Research output: Contribution to journalArticle

Futagami, Taiki ; Seto, Kazufumi ; Kajiwara, Yasuhiro ; Takashita, Hideharu ; Omori, Toshiro ; Takegawa, Kaoru ; Goto, Masatoshi. / The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans. In: Bioscience, Biotechnology and Biochemistry. 2014 ; Vol. 78, No. 2. pp. 326-335.
@article{fc23e2ba6b2741769fdbd6ef530d7bd3,
title = "The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans",
abstract = "The Mid2-like protein MtlA is a putative sensor of the cell wall integrity (CWI) signaling pathway in Aspergillus nidulans. An MtlA-EGFP fusion protein was localized at the cell surface and septa. The mtlA disruptant (ΔmtlA) showed radial colony growth similar to the wild-type (wt) strain, but showed reduced conidia formation. The ΔmtlA mutant showed growth deficiency in the presence of inhibitors of cell wall synthesis. Moreover, mtlA disruption resulted in a reduction in the glucan and chitin content in the cell wall. These results suggest that MtlA plays a significant role in asexual sporulation, cell wall stress tolerance, and the maintenance of CWI in A. nidulans, but transcriptional upregulation of α-1,3-glucan synthase gene agsB induced by micafungin was observed in the ΔmtlA strain as well as the wt strain. Thus, MtlA is not essential for activation of the downstream CWI signaling pathway components identified in previous studies of Saccharomyces cerevisiae.",
author = "Taiki Futagami and Kazufumi Seto and Yasuhiro Kajiwara and Hideharu Takashita and Toshiro Omori and Kaoru Takegawa and Masatoshi Goto",
year = "2014",
month = "1",
day = "1",
doi = "10.1080/09168451.2014.878218",
language = "English",
volume = "78",
pages = "326--335",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "2",

}

TY - JOUR

T1 - The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans

AU - Futagami, Taiki

AU - Seto, Kazufumi

AU - Kajiwara, Yasuhiro

AU - Takashita, Hideharu

AU - Omori, Toshiro

AU - Takegawa, Kaoru

AU - Goto, Masatoshi

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The Mid2-like protein MtlA is a putative sensor of the cell wall integrity (CWI) signaling pathway in Aspergillus nidulans. An MtlA-EGFP fusion protein was localized at the cell surface and septa. The mtlA disruptant (ΔmtlA) showed radial colony growth similar to the wild-type (wt) strain, but showed reduced conidia formation. The ΔmtlA mutant showed growth deficiency in the presence of inhibitors of cell wall synthesis. Moreover, mtlA disruption resulted in a reduction in the glucan and chitin content in the cell wall. These results suggest that MtlA plays a significant role in asexual sporulation, cell wall stress tolerance, and the maintenance of CWI in A. nidulans, but transcriptional upregulation of α-1,3-glucan synthase gene agsB induced by micafungin was observed in the ΔmtlA strain as well as the wt strain. Thus, MtlA is not essential for activation of the downstream CWI signaling pathway components identified in previous studies of Saccharomyces cerevisiae.

AB - The Mid2-like protein MtlA is a putative sensor of the cell wall integrity (CWI) signaling pathway in Aspergillus nidulans. An MtlA-EGFP fusion protein was localized at the cell surface and septa. The mtlA disruptant (ΔmtlA) showed radial colony growth similar to the wild-type (wt) strain, but showed reduced conidia formation. The ΔmtlA mutant showed growth deficiency in the presence of inhibitors of cell wall synthesis. Moreover, mtlA disruption resulted in a reduction in the glucan and chitin content in the cell wall. These results suggest that MtlA plays a significant role in asexual sporulation, cell wall stress tolerance, and the maintenance of CWI in A. nidulans, but transcriptional upregulation of α-1,3-glucan synthase gene agsB induced by micafungin was observed in the ΔmtlA strain as well as the wt strain. Thus, MtlA is not essential for activation of the downstream CWI signaling pathway components identified in previous studies of Saccharomyces cerevisiae.

UR - http://www.scopus.com/inward/record.url?scp=84925883258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925883258&partnerID=8YFLogxK

U2 - 10.1080/09168451.2014.878218

DO - 10.1080/09168451.2014.878218

M3 - Article

C2 - 25036689

AN - SCOPUS:84925883258

VL - 78

SP - 326

EP - 335

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -