The rat H+/K+-ATPase β subunit gene and recognition of its control region by gastric DNA binding protein

M. Maeda, K. I. Oshiman, Shigehiko Tamura, S. Kaya, S. Mahmood, M. A. Reuben, L. S. Lasater, G. Sachs, M. Futai

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The rat gastric H+/K+-ATPase β subunit gene was cloned, and its nucleotide sequence was determined. The coding region is separated by 6 introns, whereas the related human Na+/K+-ATPase β subunit gene was shown to have 5 introns (Lane, L. K., Shull, M. M., Whitmer, K. R., and Lingrel, J. B. (1989) Genomics 5, 445-453). The positions of introns 1, 2, and 5 of the two genes were the same. The similarities in intron/exon organizations and primary structures (30-40% identical residues) suggest that the β subunit genes for H+/K+- and Na+/K+-ATPases were derived from a common ancestor. The upstream region of the rat H+/K+-ATPase β subunit gene contains direct repeat sequences and palindromes, potential binding sites for RNA polymerase II and E4TF1, and CACCC box sequences. Gel retardation assay demonstrated that the stomach, but not other tissues (liver, brain, kidney, spleen, and lung), has a nuclear protein(s) capable of binding to the regions upstream of the potential RNA polymerase II binding sites (TATA box). The nuclear protein(s) are suggested to recognize three tandem GA-TAGC sequences and may be important for controlled transcription of the H+/K+-ATPase β subunit gene in gastric parietal cells.

Original languageEnglish
Pages (from-to)21584-21588
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number32
Publication statusPublished - Jan 1 1991
Externally publishedYes

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Proton-Translocating ATPases
DNA-Binding Proteins
Adenosine Triphosphatases
Rats
Stomach
Genes
Introns
RNA Polymerase II
Nuclear Proteins
H(+)-K(+)-Exchanging ATPase
Binding Sites
Gastric Parietal Cells
Inverted Repeat Sequences
TATA Box
Nucleic Acid Repetitive Sequences
Electrophoretic Mobility Shift Assay
Transcription
Genomics
Liver
Exons

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Maeda, M., Oshiman, K. I., Tamura, S., Kaya, S., Mahmood, S., Reuben, M. A., ... Futai, M. (1991). The rat H+/K+-ATPase β subunit gene and recognition of its control region by gastric DNA binding protein. Journal of Biological Chemistry, 266(32), 21584-21588.

The rat H+/K+-ATPase β subunit gene and recognition of its control region by gastric DNA binding protein. / Maeda, M.; Oshiman, K. I.; Tamura, Shigehiko; Kaya, S.; Mahmood, S.; Reuben, M. A.; Lasater, L. S.; Sachs, G.; Futai, M.

In: Journal of Biological Chemistry, Vol. 266, No. 32, 01.01.1991, p. 21584-21588.

Research output: Contribution to journalArticle

Maeda, M, Oshiman, KI, Tamura, S, Kaya, S, Mahmood, S, Reuben, MA, Lasater, LS, Sachs, G & Futai, M 1991, 'The rat H+/K+-ATPase β subunit gene and recognition of its control region by gastric DNA binding protein', Journal of Biological Chemistry, vol. 266, no. 32, pp. 21584-21588.
Maeda, M. ; Oshiman, K. I. ; Tamura, Shigehiko ; Kaya, S. ; Mahmood, S. ; Reuben, M. A. ; Lasater, L. S. ; Sachs, G. ; Futai, M. / The rat H+/K+-ATPase β subunit gene and recognition of its control region by gastric DNA binding protein. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 32. pp. 21584-21588.
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