The re-expression of nerve growth factor protein after cavity preparation in rat molars

Hisashi Shiomi, Yasuyoshi Ohsaki, Akifumi Akamine, Tadahiko Iijima

Research output: Contribution to journalArticle

Abstract

It is well known that nerve growth factor (NGF) is expressed in the odontoblasts of developing teeth, but not in completely grown teeth in rats. However, there have been no detailed studies of the expression of NGF in reactivated primary odontoblasts or in newly differentiated odontoblast-like cells during pulp repair. Therefore, to examine the involvement of NGF in odontoblasts during pulp repair, we immunohistochemically observed the localization of NGF in dental pulp after cavity preparation in rat molars. Based on the depth of the cavity, two types of pulp reaction were thus distinguished. 1) In shallow cavities, primary odontoblasts secreted a new dentin matrix, reactionary dentin, and also expressed NGF. 2) In deep cavities, odontoblast-like cells, differentiated from pulpal mesenchymal cells, formed a large amount of reparative dentin or osteodentin. Additionally, intense NGF-like immunoreactivity was observed in odontoblast-like cells. These results suggest that NGF not only acts as a neurotrophic factor of dental pulp, but is also involved in the differentiation and functional change of odontoblasts during pulp repair.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalActa Histochemica et Cytochemica
Volume33
Issue number5
DOIs
Publication statusPublished - Dec 1 2000

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Odontoblasts
Nerve Growth Factor
Rats
Pulp
Proteins
Dentin
Repair
Tooth
Dental Pulp
Nerve Growth Factors
Dental Pulp Cavity

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

The re-expression of nerve growth factor protein after cavity preparation in rat molars. / Shiomi, Hisashi; Ohsaki, Yasuyoshi; Akamine, Akifumi; Iijima, Tadahiko.

In: Acta Histochemica et Cytochemica, Vol. 33, No. 5, 01.12.2000, p. 393-398.

Research output: Contribution to journalArticle

Shiomi, Hisashi ; Ohsaki, Yasuyoshi ; Akamine, Akifumi ; Iijima, Tadahiko. / The re-expression of nerve growth factor protein after cavity preparation in rat molars. In: Acta Histochemica et Cytochemica. 2000 ; Vol. 33, No. 5. pp. 393-398.
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