Abstract
Protection against infection with Listeria monocytogenes depends primarily on Listeria-specific T cells. We show here that CD4+ TCRαβ+ T cells are capable of recognizing the mycobacterial heat shock protein (HSP) 70, that appears in the peritoneal cavity of F344 rats infected i.p. with L. monocytogenes. The HSP70-reactive CD4+ T cells recognized a peptide comprising 234-252 residues as present in the 70 kDa HSP of Mycobacterium tuberculosis in the context of RT1.B MHC class II molecules. Analysis of TCR V(β) gene expression with RT-PCR revealed that the HSP70-reactive CD4+ T cells predominantly used the V(β)16 gene segment, whereas the heat-killed Listeria (HKL)-specific T cells expressed a diverse set of V(β) gene segments. In contrast to the HKL-specific T cells producing IFN-γ, the HSP70-reactive CD4+ T cells produced TGF-β1 and IL-10 but neither T(h)1- or T(h)2-type cytokines. Adoptive transfer with HSP70-reactive T cells rendered rats susceptible to listerial infection. Collectively, these results proposed that the HSP70-reactive CD4+ T cells appearing during rat listeriosis may be involved in termination of T(h)1 cell-mediated excessive inflammation after the battle against L. monocytogenes has been won.
| Original language | English |
|---|---|
| Pages (from-to) | 117-130 |
| Number of pages | 14 |
| Journal | International Immunology |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 9 1998 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis. / Kimura, Yuki; Yamada, Katsuo; Sakai, Tetsu; Mishima, Kenichi; Nishimura, Hitoshi; Matsumoto, Yoshihiro; Singh, Mahavir; Yoshikai, Yasunobu.
In: International Immunology, Vol. 10, No. 2, 09.03.1998, p. 117-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis
AU - Kimura, Yuki
AU - Yamada, Katsuo
AU - Sakai, Tetsu
AU - Mishima, Kenichi
AU - Nishimura, Hitoshi
AU - Matsumoto, Yoshihiro
AU - Singh, Mahavir
AU - Yoshikai, Yasunobu
PY - 1998/3/9
Y1 - 1998/3/9
N2 - Protection against infection with Listeria monocytogenes depends primarily on Listeria-specific T cells. We show here that CD4+ TCRαβ+ T cells are capable of recognizing the mycobacterial heat shock protein (HSP) 70, that appears in the peritoneal cavity of F344 rats infected i.p. with L. monocytogenes. The HSP70-reactive CD4+ T cells recognized a peptide comprising 234-252 residues as present in the 70 kDa HSP of Mycobacterium tuberculosis in the context of RT1.B MHC class II molecules. Analysis of TCR V(β) gene expression with RT-PCR revealed that the HSP70-reactive CD4+ T cells predominantly used the V(β)16 gene segment, whereas the heat-killed Listeria (HKL)-specific T cells expressed a diverse set of V(β) gene segments. In contrast to the HKL-specific T cells producing IFN-γ, the HSP70-reactive CD4+ T cells produced TGF-β1 and IL-10 but neither T(h)1- or T(h)2-type cytokines. Adoptive transfer with HSP70-reactive T cells rendered rats susceptible to listerial infection. Collectively, these results proposed that the HSP70-reactive CD4+ T cells appearing during rat listeriosis may be involved in termination of T(h)1 cell-mediated excessive inflammation after the battle against L. monocytogenes has been won.
AB - Protection against infection with Listeria monocytogenes depends primarily on Listeria-specific T cells. We show here that CD4+ TCRαβ+ T cells are capable of recognizing the mycobacterial heat shock protein (HSP) 70, that appears in the peritoneal cavity of F344 rats infected i.p. with L. monocytogenes. The HSP70-reactive CD4+ T cells recognized a peptide comprising 234-252 residues as present in the 70 kDa HSP of Mycobacterium tuberculosis in the context of RT1.B MHC class II molecules. Analysis of TCR V(β) gene expression with RT-PCR revealed that the HSP70-reactive CD4+ T cells predominantly used the V(β)16 gene segment, whereas the heat-killed Listeria (HKL)-specific T cells expressed a diverse set of V(β) gene segments. In contrast to the HKL-specific T cells producing IFN-γ, the HSP70-reactive CD4+ T cells produced TGF-β1 and IL-10 but neither T(h)1- or T(h)2-type cytokines. Adoptive transfer with HSP70-reactive T cells rendered rats susceptible to listerial infection. Collectively, these results proposed that the HSP70-reactive CD4+ T cells appearing during rat listeriosis may be involved in termination of T(h)1 cell-mediated excessive inflammation after the battle against L. monocytogenes has been won.
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U2 - 10.1093/intimm/10.2.117
DO - 10.1093/intimm/10.2.117
M3 - Article
C2 - 9533439
AN - SCOPUS:0031882980
VL - 10
SP - 117
EP - 130
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 2
ER -