The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis

Yuki Kimura, Katsuo Yamada, Tetsu Sakai, Kenichi Mishima, Hitoshi Nishimura, Yoshihiro Matsumoto, Mahavir Singh, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Protection against infection with Listeria monocytogenes depends primarily on Listeria-specific T cells. We show here that CD4+ TCRαβ+ T cells are capable of recognizing the mycobacterial heat shock protein (HSP) 70, that appears in the peritoneal cavity of F344 rats infected i.p. with L. monocytogenes. The HSP70-reactive CD4+ T cells recognized a peptide comprising 234-252 residues as present in the 70 kDa HSP of Mycobacterium tuberculosis in the context of RT1.B MHC class II molecules. Analysis of TCR V(β) gene expression with RT-PCR revealed that the HSP70-reactive CD4+ T cells predominantly used the V(β)16 gene segment, whereas the heat-killed Listeria (HKL)-specific T cells expressed a diverse set of V(β) gene segments. In contrast to the HKL-specific T cells producing IFN-γ, the HSP70-reactive CD4+ T cells produced TGF-β1 and IL-10 but neither T(h)1- or T(h)2-type cytokines. Adoptive transfer with HSP70-reactive T cells rendered rats susceptible to listerial infection. Collectively, these results proposed that the HSP70-reactive CD4+ T cells appearing during rat listeriosis may be involved in termination of T(h)1 cell-mediated excessive inflammation after the battle against L. monocytogenes has been won.

    Original languageEnglish
    Pages (from-to)117-130
    Number of pages14
    JournalInternational Immunology
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - Mar 9 1998

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    Listeriosis
    HSP70 Heat-Shock Proteins
    T-Lymphocytes
    Listeria
    Listeria monocytogenes
    Hot Temperature
    Adoptive Transfer
    Peritoneal Cavity
    Inbred F344 Rats
    Infection
    Mycobacterium tuberculosis
    Interleukin-10
    Genes
    Cytokines
    Inflammation
    Gene Expression
    Polymerase Chain Reaction
    Peptides

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Kimura, Y., Yamada, K., Sakai, T., Mishima, K., Nishimura, H., Matsumoto, Y., ... Yoshikai, Y. (1998). The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis. International Immunology, 10(2), 117-130. https://doi.org/10.1093/intimm/10.2.117

    The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis. / Kimura, Yuki; Yamada, Katsuo; Sakai, Tetsu; Mishima, Kenichi; Nishimura, Hitoshi; Matsumoto, Yoshihiro; Singh, Mahavir; Yoshikai, Yasunobu.

    In: International Immunology, Vol. 10, No. 2, 09.03.1998, p. 117-130.

    Research output: Contribution to journalArticle

    Kimura, Y, Yamada, K, Sakai, T, Mishima, K, Nishimura, H, Matsumoto, Y, Singh, M & Yoshikai, Y 1998, 'The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis', International Immunology, vol. 10, no. 2, pp. 117-130. https://doi.org/10.1093/intimm/10.2.117
    Kimura Y, Yamada K, Sakai T, Mishima K, Nishimura H, Matsumoto Y et al. The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis. International Immunology. 1998 Mar 9;10(2):117-130. https://doi.org/10.1093/intimm/10.2.117
    Kimura, Yuki ; Yamada, Katsuo ; Sakai, Tetsu ; Mishima, Kenichi ; Nishimura, Hitoshi ; Matsumoto, Yoshihiro ; Singh, Mahavir ; Yoshikai, Yasunobu. / The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis. In: International Immunology. 1998 ; Vol. 10, No. 2. pp. 117-130.
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