The relationship between the adenine nucleotide metabolism and the conversion of the xanthine oxidase enzyme system in ischemia-reperfusion of the rat small intestine

Yuzo Hirata, Tomoaki Taguchi, Makoto Nakao, Takaharu Yamada, Ryuichiro Hirose, Sachiyo Suita

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The time course of the energy metabolism after reperfusion, the relationship between the conversion of xanthine dehydrogenase to xanthine oxidase (D-to-O conversion) during ischemia, and the changes of the energy metabolism after reperfusion were studied using an ischemia-reperfusion model in the small intestine of the rat. The rat jejunum underwent an occlusion of the superior mesenteric artery and vein for either 30 minutes (group 1, n = 6) or 90 minutes (group 2, n = 6) with collateral interruption, and then it was reperfused. The contents of the adenine nucleotides in the small intestine of the rat were measured by high-performance liquid chromatography (HPLC) before ischemia, and 30, 60, and 90 minutes of ischemia, as well as 30, 60, 120, and 180 minutes after reperfusion. The recovery level of adenosine triphosphate (ATP) in group 1 (6.05 ± 0.80 μmol/g dry weight) 30 minutes after reperfusion was significantly higher than that in group 2 (2.28 ± 1.12 μmol/g dry weight) (P < .001). In addition, the ATP content after reperfusion in group 2 did not change from 30 to 180 minutes after reperfusion. The D-to-O conversion during ischemia in group 1 was not significantly greater than that before ischemia; however, that of group 2 did increase significantly during ischemia (P < .005). These results suggest that the tissue damage from ischemia-reperfusion injury after reperfusion under 90 minutes' ischemia is accomplished within the first 30 minutes after reperfusion. Therefore, the ATP level at 30 minutes after reperfusion may be useful for the evaluation of intestinal viability. Thus, the conversion of the xanthine oxidase enzyme system might play an important role in the expression of ischemia-reperfusion injury.

Original languageEnglish
Pages (from-to)1199-1204
Number of pages6
JournalJournal of Pediatric Surgery
Volume31
Issue number9
DOIs
Publication statusPublished - Sep 1996

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Adenine Nucleotides
Xanthine Oxidase
Small Intestine
Reperfusion
Ischemia
Enzymes
Adenosine Triphosphate
Reperfusion Injury
Energy Metabolism
Xanthine Dehydrogenase
Weights and Measures
Mesenteric Veins
Superior Mesenteric Artery
Jejunum
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

The relationship between the adenine nucleotide metabolism and the conversion of the xanthine oxidase enzyme system in ischemia-reperfusion of the rat small intestine. / Hirata, Yuzo; Taguchi, Tomoaki; Nakao, Makoto; Yamada, Takaharu; Hirose, Ryuichiro; Suita, Sachiyo.

In: Journal of Pediatric Surgery, Vol. 31, No. 9, 09.1996, p. 1199-1204.

Research output: Contribution to journalArticle

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