TY - JOUR
T1 - The rice GRH2 and GRH4 activate various defense responses to the green rice leafhopper and confer strong insect resistance
AU - Asano, Takayuki
AU - Tamura, Yasumori
AU - Yasui, Hiroe
AU - Satoh, Kouji
AU - Hattori, Makoto
AU - Yasui, Hideshi
AU - Kikuchi, Shoshi
N1 - Publisher Copyright:
© 2015 The Japanese Society for Plant Cell and Molecular Biology.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2015/10/29
Y1 - 2015/10/29
N2 - fonfers enhanced resistance to green rice leafhoppers (GRH), Nephotettix cincticeps Uhler. A pyramided line carrying GRH2 and GRH4 (TGRH29) showed strong resistance to GRH insects compared with a GRH2 near-isogenic line (TGRH11), although GRH4 alone did not confer any resistance to GRH. To explore the effects of GRH2 and GRH4 on GRH resistance, we investigated the transcriptional response of rice plants to GRH infestation using DNA microarray analysis. The expression of a large number of genes encoding pathogenesis-related proteins, lipoxygenases, terpene synthase (TPS) and WRKY transcription factor, was upregulated in response to GRH infestation in TGRH11 and TGRH29 compared with control plants. Quantitative RT-PCR revealed that expression of JAmyb and TPS was more strongly and more rapidly upregulated in TGRH29 compared with TGRH11 after GRH infestation. These results suggest that TGRH29 plants can more rapidly and strongly activate the defense response compared with plants carrying GRH2 alone. Furthermore, sesquiterpenes were emitted from TGRH29 plants in response to attack by GRH. The strong induction of sesquiterpene production in the TGRH29 line was correlated with the transcript levels of TPS genes. Our results suggest that GRH2 and GRH4 activate various defense responses and confer strong GRH insect resistance.
AB - fonfers enhanced resistance to green rice leafhoppers (GRH), Nephotettix cincticeps Uhler. A pyramided line carrying GRH2 and GRH4 (TGRH29) showed strong resistance to GRH insects compared with a GRH2 near-isogenic line (TGRH11), although GRH4 alone did not confer any resistance to GRH. To explore the effects of GRH2 and GRH4 on GRH resistance, we investigated the transcriptional response of rice plants to GRH infestation using DNA microarray analysis. The expression of a large number of genes encoding pathogenesis-related proteins, lipoxygenases, terpene synthase (TPS) and WRKY transcription factor, was upregulated in response to GRH infestation in TGRH11 and TGRH29 compared with control plants. Quantitative RT-PCR revealed that expression of JAmyb and TPS was more strongly and more rapidly upregulated in TGRH29 compared with TGRH11 after GRH infestation. These results suggest that TGRH29 plants can more rapidly and strongly activate the defense response compared with plants carrying GRH2 alone. Furthermore, sesquiterpenes were emitted from TGRH29 plants in response to attack by GRH. The strong induction of sesquiterpene production in the TGRH29 line was correlated with the transcript levels of TPS genes. Our results suggest that GRH2 and GRH4 activate various defense responses and confer strong GRH insect resistance.
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U2 - 10.5511/plantbiotechnology.15.0615a
DO - 10.5511/plantbiotechnology.15.0615a
M3 - Article
AN - SCOPUS:84945962349
SN - 1342-4580
VL - 32
SP - 215
EP - 224
JO - Plant Biotechnology
JF - Plant Biotechnology
IS - 3
ER -