TY - JOUR
T1 - The role of γδ T cells in priming macrophages to produce tumor necrosis factor‐α
AU - Nishimura, Hitoshi
AU - Emoto, Masashi
AU - Hiromatsu, Kenji
AU - Yamamoto, Shunsuke
AU - Matsuura, Keiko
AU - Gomi, Hiroshi
AU - Ikeda, Toshio
AU - Itohara, Shigeyoshi
AU - Yoshikai, Yasunobu
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1995/5
Y1 - 1995/5
N2 - The secretion of tumor necrosis factor (TNF)‐α from macrophages is regulated by both priming and triggering signals. We found that macrophages from mice lacking γδ T cells [T cell receptor (TCR) δ−/‐ mice], which lack the gene encoding the δ chain, produced only small amounts of TNF‐α in response to lipopolysaccharide (LPS) and showed a reduced level of expression of CD14. Pre‐incubation of macrophages from TCR δ‐/‐ mice with γδ T cells from their TCR δ+/‐ littermates restored their capacity to produce TNF‐α in response to LPS. The priming activity of γδ T cells was in part inhibited by neutralizing anti‐interferon (IFN)‐γ monoclonal antibodies. Collectively, these results suggest that γδ T cells play a role in priming macrophages to a steady state of activation via IFN‐γ secretion, which allows them to produce TNF‐α when exposed to LPS.
AB - The secretion of tumor necrosis factor (TNF)‐α from macrophages is regulated by both priming and triggering signals. We found that macrophages from mice lacking γδ T cells [T cell receptor (TCR) δ−/‐ mice], which lack the gene encoding the δ chain, produced only small amounts of TNF‐α in response to lipopolysaccharide (LPS) and showed a reduced level of expression of CD14. Pre‐incubation of macrophages from TCR δ‐/‐ mice with γδ T cells from their TCR δ+/‐ littermates restored their capacity to produce TNF‐α in response to LPS. The priming activity of γδ T cells was in part inhibited by neutralizing anti‐interferon (IFN)‐γ monoclonal antibodies. Collectively, these results suggest that γδ T cells play a role in priming macrophages to a steady state of activation via IFN‐γ secretion, which allows them to produce TNF‐α when exposed to LPS.
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U2 - 10.1002/eji.1830250551
DO - 10.1002/eji.1830250551
M3 - Article
C2 - 7539762
AN - SCOPUS:0029071697
VL - 25
SP - 1465
EP - 1468
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 5
ER -