The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation: The HyHEL-10-HEL interaction

Akiko Yokota, Kouhei Tsumoto, Mitsunori Shiroishi, Hidemasa Kondo, Izumi Kumagai

Research output: Contribution to journalArticle

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Abstract

To study the role of hydrogen bonding via interfacial water molecules in protein-protein interactions, we examined the interaction between hen egg white lysozyme (HEL) and its HyHEL-10 variable domain fragment (Fv) antibody. We constructed three antibody mutants (L-Y50F, L-S91A, and L-S93A) and investigated the interactions between the mutant Fvs and HEL. Isothermal titration calorimetry indicated that the mutations significantly decreased the negative enthalpy change (8-25 kJ mol-1), despite some offset by a favorable entropy change. X-ray crystallography demonstrated that the complexes had nearly identical structures, including the positions of the interfacial water molecules. Taken together, the isothermal titration calorimetric and x-ray crystallographic results indicate that hydrogen bonding via interfacial water enthalpically contributes to the Fv-HEL interaction despite the partial offset because of entropy loss, suggesting that hydrogen bonding stiffens the antigen-antibody complex.

Original languageEnglish
Pages (from-to)5410-5418
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number7
DOIs
Publication statusPublished - Feb 14 2003

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Egg White
Hydrogen Bonding
Muramidase
Complexation
Hydrogen bonds
Entropy
Titration
Antigens
Molecules
Water
Antibodies
Immunoglobulin Variable Region
Calorimetry
X ray crystallography
X Ray Crystallography
Antigen-Antibody Complex
Enthalpy
Proteins
X-Rays
X rays

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction. / Yokota, Akiko; Tsumoto, Kouhei; Shiroishi, Mitsunori; Kondo, Hidemasa; Kumagai, Izumi.

In: Journal of Biological Chemistry, Vol. 278, No. 7, 14.02.2003, p. 5410-5418.

Research output: Contribution to journalArticle

Yokota, Akiko ; Tsumoto, Kouhei ; Shiroishi, Mitsunori ; Kondo, Hidemasa ; Kumagai, Izumi. / The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 7. pp. 5410-5418.
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