The role of NF-kB in retinal neovascularization in the rat: Possible involvement of cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin-8 family

Ayako Yoshida, Shigeo Yoshida, Yasuaki Hata, Ahmad K. Khalil, Tatsuro Ishibashi, Hajime Inomata

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Hypoxia precedes neovascularization in many retinal diseases that can lead to irreversible vision loss. The transcription factor NF-KB is activated by hypoxia and regulates the expression of many genes, including angiogenic factors. The relation between the NF-kB activation and the cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin-8 (IL-8) family, was investigated by immunohistochemistry in a rat model of proliferative retinopathy presumably caused by relative hypoxia. Activated NF-KB and CINC immunoreactivity was detected in retinal glial cells in the nonperfused retina and in neovascular cells. Activated NF-kB was detected before the CINC staining, and both of these events occurred before the development of neovascularization. The intensity of both activated NF-KB and CINC staining remained increased during the development of neovascularization and then declined as neovascularization regressed. In rat retinal glial cells in vitro, dexamethasone, an inhibitor of NF-kB activation, prevented the hypoxia-induced increase in the amount of CINC mRNA. Furthermore, CINC induced neovascularization in a rat corneal pocket model. These results suggest that hypoxia-induced activation of NF-kB results in CINC production and participates in the induction of retinal neovascularization.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume46
Issue number4
DOIs
Publication statusPublished - Apr 1998

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

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