The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium

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Abstract

Background: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. Purpose: We investigated the mechanism by which OECs adhere to titanium dental implants. Materials and methods: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. Results: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. Conclusion: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.

Original languageEnglish
Pages (from-to)1696-1708
Number of pages13
JournalArchives of Oral Biology
Volume58
Issue number11
DOIs
Publication statusPublished - Oct 4 2013

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1-Phosphatidylinositol 4-Kinase
Titanium
Epithelial Cells
Integrins
Intercellular Signaling Peptides and Proteins
Experimental Implants
Dental Implants
Somatomedins
Cell Adhesion
Cell Movement
Actins
Epithelium

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

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title = "The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium",
abstract = "Background: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. Purpose: We investigated the mechanism by which OECs adhere to titanium dental implants. Materials and methods: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. Results: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. Conclusion: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.",
author = "Ikiru Atsuta and Yasunori Ayukawa and Takayoshi Yamaza and Akihiro Furuhashi and Kiyoshi Koyano",
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T1 - The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium

AU - Atsuta, Ikiru

AU - Ayukawa, Yasunori

AU - Yamaza, Takayoshi

AU - Furuhashi, Akihiro

AU - Koyano, Kiyoshi

PY - 2013/10/4

Y1 - 2013/10/4

N2 - Background: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. Purpose: We investigated the mechanism by which OECs adhere to titanium dental implants. Materials and methods: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. Results: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. Conclusion: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.

AB - Background: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. Purpose: We investigated the mechanism by which OECs adhere to titanium dental implants. Materials and methods: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. Results: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. Conclusion: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.

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