TY - JOUR
T1 - The roles of angiotensin II in stretched periodontal ligament cells
AU - Monnouchi, S.
AU - Maeda, H.
AU - Fujii, S.
AU - Tomokiyo, A.
AU - Kono, K.
AU - Akamine, A.
N1 - Funding Information:
This work was supported by grants-in-aid (projects 21390510, 21791942, 22390359, and 22592123) for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan. The author(s) declare no potential conflicts of interests with respect to the authorship and/or publication of this article.
PY - 2011/2
Y1 - 2011/2
N2 - The loading caused by occlusion and mastication plays an important role in maintaining periodontal ligament (PDL) tissues. We hypothesized that a loading magnitude would be involved in the production of biological factors that function in the maintenance of PDL tissues. Here, we identified up-regulated gene expressions of transforming growth factor-Î1 (TGF-Î1), alkaline phosphatase (ALP), and angiotensinogen in human PDL fibroblastic cells (HPLFs) that were exposed to 8% stretch loading. Immunolocalization of angiotensin I/II (Ang I/II), which was converted from angiotensinogen, was detected in rat PDL tissues. HPLFs that were stimulated by Ang II also increased their gene expressions of TGF-Î1 and ALP. Furthermore, the antagonist for Ang II type 2 receptor, rather than for type 1, significantly inhibited gene expressions induced by the stretch loading. Analysis of these data suggests that Ang II mediates the loading signal in stretched HPLFs to induce expressions of TGF-Î1 and ALP.
AB - The loading caused by occlusion and mastication plays an important role in maintaining periodontal ligament (PDL) tissues. We hypothesized that a loading magnitude would be involved in the production of biological factors that function in the maintenance of PDL tissues. Here, we identified up-regulated gene expressions of transforming growth factor-Î1 (TGF-Î1), alkaline phosphatase (ALP), and angiotensinogen in human PDL fibroblastic cells (HPLFs) that were exposed to 8% stretch loading. Immunolocalization of angiotensin I/II (Ang I/II), which was converted from angiotensinogen, was detected in rat PDL tissues. HPLFs that were stimulated by Ang II also increased their gene expressions of TGF-Î1 and ALP. Furthermore, the antagonist for Ang II type 2 receptor, rather than for type 1, significantly inhibited gene expressions induced by the stretch loading. Analysis of these data suggests that Ang II mediates the loading signal in stretched HPLFs to induce expressions of TGF-Î1 and ALP.
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U2 - 10.1177/0022034510382118
DO - 10.1177/0022034510382118
M3 - Article
C2 - 21270461
AN - SCOPUS:79951634520
SN - 0022-0345
VL - 90
SP - 181
EP - 185
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 2
ER -