The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation

Mitsuaki Tabuchi, Naotaka Tanaka, Shojiro Iwahara, Kaoru Takegawa

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

In a previous study, we isolated a Schizosaccharomyces pombe mutant defective in protein galactosylation. From an S. pombe genomic library, we cloned the gms1+ gene which restored the galactosylation of cell wall glycoproteins. Gms1 protein shares significant sequence similarity with human UDP-galactose and murine CMP-sialic acid transporters. The fission yeast strains deleted for the gms1+ gene lacked galactose residues in sell surface glycoproteins and were significantly decreased in UDP-galactose transport activity. These results showed that the gms1+ encodes an UDP-galactose transporter, and this protein appears to be an essential role for the incorporation of UDP-galactose into the lumen of Golgi in S. pombe.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume232
Issue number1
DOIs
Publication statusPublished - Mar 6 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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