The significance of carbonic anhydrase expression in human colorectal cancer

Masaki Mori, Raymond J. Staniunas, Graham F. Barnard, J. Milburn Jessup, Glenn D. Steele, Lan Bo Chen

Research output: Contribution to journalArticle

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Abstract

Background: Carbonic anhydrase isoenzymes I and II are present in normal colorectal mucosa. This study aimed to determine if carbonic anhydrase is present in colorectal cancer and what is its potential clinical significance. Methods: The messenger RNA (mRNA) and protein expression of carbonic anhydrase isoenzyme I were analyzed in fresh specimens of colorectal adenocarcinoma by Northern and Western blots, respectively. The immunohistochemical expression was subsequently studied in a larger number of formalin-fixed surgical specimens. Results: All of 30 normal colon samples had a strong RNA hybridization signal. Only 4 of 39 paired colorectal cancer and none of 9 normal liver samples had detectable levels of carbonic anhydrase mRNA. Isoenzyme I protein expression showed similar results. In a separate group of patients, immunohistochemical studies showed that 16 of 96 colorectal tumors had positive staining cells. All positive tumors were well or moderately differentiated carcinomas (P < 0.05). When analyzed retrospectively, immunoreactive cases were more likely to be in a group with a good outcome (P < 0.01) and to lack vascular invasion (P < 0.01) than negative cases. Conclusions: The majority of colorectal cancers do not express carbonic anhydrase isoenzyme I. The presence of any isoenzyme I-positive immunoreactive cancer cells may be associated with a more favorable outcome in colorectal cancer.

Original languageEnglish
Pages (from-to)820-826
Number of pages7
JournalGastroenterology
Volume105
Issue number3
DOIs
Publication statusPublished - Sep 1993

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Carbonic Anhydrases
Isoenzymes
Carbonic Anhydrase I
Colorectal Neoplasms
Carbonic Anhydrase II
Messenger RNA
Northern Blotting
Formaldehyde
Blood Vessels
Neoplasms
Colon
Mucous Membrane
Proteins
Adenocarcinoma
Western Blotting
RNA
Staining and Labeling
Carcinoma
Liver

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Mori, M., Staniunas, R. J., Barnard, G. F., Jessup, J. M., Steele, G. D., & Chen, L. B. (1993). The significance of carbonic anhydrase expression in human colorectal cancer. Gastroenterology, 105(3), 820-826. https://doi.org/10.1016/0016-5085(93)90900-W

The significance of carbonic anhydrase expression in human colorectal cancer. / Mori, Masaki; Staniunas, Raymond J.; Barnard, Graham F.; Jessup, J. Milburn; Steele, Glenn D.; Chen, Lan Bo.

In: Gastroenterology, Vol. 105, No. 3, 09.1993, p. 820-826.

Research output: Contribution to journalArticle

Mori, M, Staniunas, RJ, Barnard, GF, Jessup, JM, Steele, GD & Chen, LB 1993, 'The significance of carbonic anhydrase expression in human colorectal cancer', Gastroenterology, vol. 105, no. 3, pp. 820-826. https://doi.org/10.1016/0016-5085(93)90900-W
Mori, Masaki ; Staniunas, Raymond J. ; Barnard, Graham F. ; Jessup, J. Milburn ; Steele, Glenn D. ; Chen, Lan Bo. / The significance of carbonic anhydrase expression in human colorectal cancer. In: Gastroenterology. 1993 ; Vol. 105, No. 3. pp. 820-826.
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