TY - JOUR
T1 - The significance of oral streptococci in patients with pneumonia with risk factors for aspiration
T2 - The bacterial floral analysis of 16S ribosomal RNA gene using bronchoalveolar lavage fluid
AU - Akata, Kentaro
AU - Yatera, Kazuhiro
AU - Yamasaki, Kei
AU - Kawanami, Toshinori
AU - Naito, Keisuke
AU - Noguchi, Shingo
AU - Fukuda, Kazumasa
AU - Ishimoto, Hiroshi
AU - Taniguchi, Hatsumi
AU - Mukae, Hiroshi
N1 - Funding Information:
We thank Drs. Yukikazu Awaya, Chiharu Yoshii, Hideto Obata, Yukiko Kawanami, Yugo Yoshida, Takashi Kido, Takeshi Orihashi, Chinatsu Nishida, Naoyuki Inoue, Takaaki Ogoshi, Susumu Tokuyama, Minako Hanaka, Yu Suzuki, Keishi Oda, Kanako Hara and Tetsuya Hanaka for collecting clinical data, and Ms. Yoshiko Yamazaki, Michiyo Taguchi, Kumiko Matsuyama for their technical supports. This study was partially supported by a High Altitude Research Grant from the University of Occupational and Environmental Health, Japan, and a Research Grant from the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (C), 23591173, 2011.
Publisher Copyright:
© 2016 Akata et al.
PY - 2016/5/11
Y1 - 2016/5/11
N2 - Background: Aspiration pneumonia has been a growing interest in an aging population. Anaerobes are important pathogens, however, the etiology of aspiration pneumonia is not fully understood. In addition, the relationship between the patient clinical characteristics and the causative pathogens in pneumonia patients with aspiration risk factors are unclear. To evaluate the relationship between the patient clinical characteristics with risk factors for aspiration and bacterial flora in bronchoalveolar lavage fluid (BALF) in pneumonia patients, the bacterial floral analysis of 16S ribosomal RNA gene was applied in addition to cultivation methods in BALF samples. Methods: From April 2010 to February 2014, BALF samples were obtained from the affected lesions of pneumonia via bronchoscopy, and were evaluated by the bacterial floral analysis of 16S rRNA gene in addition to cultivation methods in patients with community-acquired pneumonia (CAP) and healthcare-associated pneumonia (HCAP). Factors associated with aspiration risks in these patients were analyzed. Results: A total of 177 (CAP 83, HCAP 94) patients were enrolled. According to the results of the bacterial floral analysis, detection rate of oral streptococci as the most detected bacterial phylotypes in BALF was significantly higher in patients with aspiration risks (31.0 %) than in patients without aspiration risks (14.7 %) (P = 0.009). In addition, the percentages of oral streptococci in each BALF sample were significantly higher in patients with aspiration risks (26.6 ± 32.0 %) than in patients without aspiration risks (13.8 ± 25.3 %) (P = 0.002). A multiple linear regression analysis showed that an Eastern Cooperative Oncology Group (ECOG) performance status (PS) of ≥3, the presence of comorbidities, and a history of pneumonia within a previous year were significantly associated with a detection of oral streptococci in BALF. Conclusions: The bacterial floral analysis of 16S rRNA gene revealed that oral streptococci were mostly detected as the most detected bacterial phylotypes in BALF samples in CAP and HCAP patients with aspiration risks, especially in those with a poor ECOG-PS or a history of pneumonia.
AB - Background: Aspiration pneumonia has been a growing interest in an aging population. Anaerobes are important pathogens, however, the etiology of aspiration pneumonia is not fully understood. In addition, the relationship between the patient clinical characteristics and the causative pathogens in pneumonia patients with aspiration risk factors are unclear. To evaluate the relationship between the patient clinical characteristics with risk factors for aspiration and bacterial flora in bronchoalveolar lavage fluid (BALF) in pneumonia patients, the bacterial floral analysis of 16S ribosomal RNA gene was applied in addition to cultivation methods in BALF samples. Methods: From April 2010 to February 2014, BALF samples were obtained from the affected lesions of pneumonia via bronchoscopy, and were evaluated by the bacterial floral analysis of 16S rRNA gene in addition to cultivation methods in patients with community-acquired pneumonia (CAP) and healthcare-associated pneumonia (HCAP). Factors associated with aspiration risks in these patients were analyzed. Results: A total of 177 (CAP 83, HCAP 94) patients were enrolled. According to the results of the bacterial floral analysis, detection rate of oral streptococci as the most detected bacterial phylotypes in BALF was significantly higher in patients with aspiration risks (31.0 %) than in patients without aspiration risks (14.7 %) (P = 0.009). In addition, the percentages of oral streptococci in each BALF sample were significantly higher in patients with aspiration risks (26.6 ± 32.0 %) than in patients without aspiration risks (13.8 ± 25.3 %) (P = 0.002). A multiple linear regression analysis showed that an Eastern Cooperative Oncology Group (ECOG) performance status (PS) of ≥3, the presence of comorbidities, and a history of pneumonia within a previous year were significantly associated with a detection of oral streptococci in BALF. Conclusions: The bacterial floral analysis of 16S rRNA gene revealed that oral streptococci were mostly detected as the most detected bacterial phylotypes in BALF samples in CAP and HCAP patients with aspiration risks, especially in those with a poor ECOG-PS or a history of pneumonia.
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U2 - 10.1186/s12890-016-0235-z
DO - 10.1186/s12890-016-0235-z
M3 - Article
C2 - 27169775
AN - SCOPUS:84968735492
SN - 1471-2466
VL - 16
JO - BMC Pulmonary Medicine
JF - BMC Pulmonary Medicine
IS - 1
M1 - 79
ER -