TY - JOUR
T1 - The significance of serum active matrix metalloproteinase-9 in patients with non-small cell lung cancer
AU - Ondo, Kaoru
AU - Sugio, Kenji
AU - Yamazaki, Koji
AU - Yamaguchi, Masafumi
AU - Yano, Tokujiro
AU - Yoshino, Ichiro
AU - Maehara, Yoshihiko
N1 - Funding Information:
We thank Dr. Brian T. Quinn for critical comments. This work was supported in part by a Grand-in-Aid for Scientific Research (14571292) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
PY - 2004/11
Y1 - 2004/11
N2 - Matrix metalloproteinase-9 (MMP-9) plays an essential role for metastasis. The purpose of this study is to investigate the significance of active MMP-9 quantified in serum of patients with non-small cell lung cancer. Immunohistochemical staining of the primary tumors was also performed to analyze how much the secretion of MMP-9 from cancer cells influences the concentration of MMP-9 in serum. The preoperative sera of 39 patients with NSCLC, who underwent an operation at our institution from 1996 to 1999, were taken before the operation. Forty-three samples of sera were drawn postoperatively. Of these 43 samples, 16 sera were taken from patients who suffered with recurrence, 27 sera were taken from patients who did not suffer with recurrence. The serum level of active MMP-9 of preoperative state was 24.3 ± 21.2 ng/ml, which was higher than that of control was 14.6 ± 18.8 ng/ml, but no significance was recognized statistically between them (P = 0.0666). The immunoreactivity of MMP-9 was not correlated statistically with serum level of total MMP-9 and active MMP-9. The activity ratio of non-recurrent group was also a lower level than that of recurrent group (P = 0.0082). In conclusion, our results suggest that the concentration of serum active MMP-9 has a possibility of being an available marker to detect a recurrent disease. Our findings strongly suggest that measuring the activity ratio of serum MMP-9 appears to be a potentially useful tumor maker.
AB - Matrix metalloproteinase-9 (MMP-9) plays an essential role for metastasis. The purpose of this study is to investigate the significance of active MMP-9 quantified in serum of patients with non-small cell lung cancer. Immunohistochemical staining of the primary tumors was also performed to analyze how much the secretion of MMP-9 from cancer cells influences the concentration of MMP-9 in serum. The preoperative sera of 39 patients with NSCLC, who underwent an operation at our institution from 1996 to 1999, were taken before the operation. Forty-three samples of sera were drawn postoperatively. Of these 43 samples, 16 sera were taken from patients who suffered with recurrence, 27 sera were taken from patients who did not suffer with recurrence. The serum level of active MMP-9 of preoperative state was 24.3 ± 21.2 ng/ml, which was higher than that of control was 14.6 ± 18.8 ng/ml, but no significance was recognized statistically between them (P = 0.0666). The immunoreactivity of MMP-9 was not correlated statistically with serum level of total MMP-9 and active MMP-9. The activity ratio of non-recurrent group was also a lower level than that of recurrent group (P = 0.0082). In conclusion, our results suggest that the concentration of serum active MMP-9 has a possibility of being an available marker to detect a recurrent disease. Our findings strongly suggest that measuring the activity ratio of serum MMP-9 appears to be a potentially useful tumor maker.
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U2 - 10.1016/j.lungcan.2004.04.030
DO - 10.1016/j.lungcan.2004.04.030
M3 - Article
C2 - 15474669
AN - SCOPUS:4944234684
SN - 0169-5002
VL - 46
SP - 205
EP - 213
JO - Lung Cancer
JF - Lung Cancer
IS - 2
ER -
