The specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro.

M. Furuichi, N. Hamada, O. Nakamura, A. Razzaque, J. I. Mukai, S. Murao, T. Nishino

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Abstract

All the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by Streptomyces adephospholyticus ATP:nucleotide pyrophosphokinase (E.C.2.7.6.4) form dATP to the respective 5'-phosphates, and their effects on the translation of mRNAs by a wheat germ system in vitro were studied. (p) ppPupp decreased the total 14C-leucine incorporation directed by a rat liver mRNA whereas (p) ppPypp did not. With a silkworm pupa ovary mRNA, distinctly reverase results were obtained. Gel electrophoretic profiles of the translation products disclosed the mRNA species-specific stimulatory or inhibitory effects for each of the polyphosphates tested.

Original languageEnglish
Pages (from-to)s151-154
JournalNucleic acids symposium series
Issue number6
Publication statusPublished - Dec 1 1979
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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