The specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro.

M. Furuichi, N. Hamada, O. Nakamura, A. Razzaque, J. I. Mukai, S. Murao, T. Nishino

Research output: Contribution to journalArticle

Abstract

All the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by Streptomyces adephospholyticus ATP:nucleotide pyrophosphokinase (E.C.2.7.6.4) form dATP to the respective 5'-phosphates, and their effects on the translation of mRNAs by a wheat germ system in vitro were studied. (p) ppPupp decreased the total 14C-leucine incorporation directed by a rat liver mRNA whereas (p) ppPypp did not. With a silkworm pupa ovary mRNA, distinctly reverase results were obtained. Gel electrophoretic profiles of the translation products disclosed the mRNA species-specific stimulatory or inhibitory effects for each of the polyphosphates tested.

Original languageEnglish
Pages (from-to)s151-154
JournalNucleic acids symposium series
Issue number6
Publication statusPublished - Dec 1 1979

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Pyrimidine Nucleosides
Purine Nucleosides
Diphosphates
nucleotide pyrophosphokinase
Messenger RNA
Ribonucleosides
Polyphosphates
Pupa
Bombyx
Streptomyces
Protein Biosynthesis
Leucine
Triticum
Ovary
Gels
Phosphates
Liver
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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The specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro. / Furuichi, M.; Hamada, N.; Nakamura, O.; Razzaque, A.; Mukai, J. I.; Murao, S.; Nishino, T.

In: Nucleic acids symposium series, No. 6, 01.12.1979, p. s151-154.

Research output: Contribution to journalArticle

Furuichi, M. ; Hamada, N. ; Nakamura, O. ; Razzaque, A. ; Mukai, J. I. ; Murao, S. ; Nishino, T. / The specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro. In: Nucleic acids symposium series. 1979 ; No. 6. pp. s151-154.
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