The specific uptake of cloned Haemophilus DNA

Bon Chu Chung, Sol H. Goodgal

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the process of transformation Haemophilusinfluenzae cells bind its own DNA but little or no foreign DNA. This specificity for recognition of DNA was studied by cloning Haemophilus DNA in E. coli. Haemophilus DNA fragments were cloned using plasmid pBR322 as a vector. The fragment cH7 cloned in pBR322 was found to be homologous to Haemophilus DNA and shown to bind irreversibly to competent Haemophilus cells. The fact that cH7 isolated from E. coli lacks Haemophilus modification leads to the conclusion that modification does not play a role in the uptake mechanism. Uptake specificity is a function of recognition sequences that reside in DNA itself.

Original languageEnglish
Pages (from-to)208-214
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume88
Issue number1
DOIs
Publication statusPublished - May 14 1979

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Haemophilus
DNA
Escherichia coli
Cloning
Organism Cloning
Plasmids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The specific uptake of cloned Haemophilus DNA. / Chung, Bon Chu; Goodgal, Sol H.

In: Biochemical and Biophysical Research Communications, Vol. 88, No. 1, 14.05.1979, p. 208-214.

Research output: Contribution to journalArticle

Chung, Bon Chu ; Goodgal, Sol H. / The specific uptake of cloned Haemophilus DNA. In: Biochemical and Biophysical Research Communications. 1979 ; Vol. 88, No. 1. pp. 208-214.
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