The stage of negative selection in tolerance induction in neonatal mice

T. Ando, Y. Yoshikai, G. Matsuzaki, H. Takimoto, K. Nomoto

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mls(b/a)) lymphoid cells, both CD4+ and CD8+ cells showed a striking reduction in the number of Vβ6+ cells capable of recognizing Mlsa in the context of major histocompatibility complex (MHC) class II molecules, indicating that clonal deletion of Vβ6+ cells by Mlsa antigen occurs just at a stage of immature Vβ6(low)CD4+CD8+ thymocytes. On the other hand, the number of Vβ11+ cells capable of recognizing I-E was markedly reduced in CD4+ cells, but CD8+ cells showed only partial (20%) reduction of such a population. The clonal deletion of Vβ11+ cells by I-E may begin at the transitional stage from Vβ11(low)CD4+CD8+ to Vβ11(high)CD4+CD8- single-position cells, and Vβ11(low)CD4+CD8+ cells differentiating to Vβ11(high)CD4-CD8+ cells seem to be resistant to clonal deletion. Vβ11+ T cells are also stimulated by staphylococcal enterotoxin A (SEA) irrespective of expression of CD4 or CD8. Nearly all of both Vβ11+CD4+ and Vβ11+CD8+ lymph node T cells were deleted by the injection of SEA every other day from birth. In their thymi, both Vβ11+CD4+CD8- and Vβ11+CD4-CD8+ single-positive thymocytes were deleted, and the proportion of Vβ11(low) thymocytes was lower than that of normal mice. The clonal deletion of Vβ11+ T cells by SEA injection occurs at a stage of immature Vβ11(low)CD4+CD8+ double-positive thymocytes, resulting in deletion of both Vβ11+CD4+ and Vβ11+CD8+ T cells.

    Original languageEnglish
    Pages (from-to)638-644
    Number of pages7
    JournalImmunology
    Volume74
    Issue number4
    Publication statusPublished - 1991

    Fingerprint

    Clonal Deletion
    Thymocytes
    T-Lymphocytes
    Thymus Gland
    Cell Count
    Lymph Nodes
    Injections
    Major Histocompatibility Complex
    Flow Cytometry
    Parturition
    Lymphocytes
    Antigens
    Population
    Staphylococcal enterotoxin A

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Ando, T., Yoshikai, Y., Matsuzaki, G., Takimoto, H., & Nomoto, K. (1991). The stage of negative selection in tolerance induction in neonatal mice. Immunology, 74(4), 638-644.

    The stage of negative selection in tolerance induction in neonatal mice. / Ando, T.; Yoshikai, Y.; Matsuzaki, G.; Takimoto, H.; Nomoto, K.

    In: Immunology, Vol. 74, No. 4, 1991, p. 638-644.

    Research output: Contribution to journalArticle

    Ando, T, Yoshikai, Y, Matsuzaki, G, Takimoto, H & Nomoto, K 1991, 'The stage of negative selection in tolerance induction in neonatal mice', Immunology, vol. 74, no. 4, pp. 638-644.
    Ando T, Yoshikai Y, Matsuzaki G, Takimoto H, Nomoto K. The stage of negative selection in tolerance induction in neonatal mice. Immunology. 1991;74(4):638-644.
    Ando, T. ; Yoshikai, Y. ; Matsuzaki, G. ; Takimoto, H. ; Nomoto, K. / The stage of negative selection in tolerance induction in neonatal mice. In: Immunology. 1991 ; Vol. 74, No. 4. pp. 638-644.
    @article{d7e5d0efc4074f6f92df96a0a85e102e,
    title = "The stage of negative selection in tolerance induction in neonatal mice",
    abstract = "In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mls(b/a)) lymphoid cells, both CD4+ and CD8+ cells showed a striking reduction in the number of Vβ6+ cells capable of recognizing Mlsa in the context of major histocompatibility complex (MHC) class II molecules, indicating that clonal deletion of Vβ6+ cells by Mlsa antigen occurs just at a stage of immature Vβ6(low)CD4+CD8+ thymocytes. On the other hand, the number of Vβ11+ cells capable of recognizing I-E was markedly reduced in CD4+ cells, but CD8+ cells showed only partial (20{\%}) reduction of such a population. The clonal deletion of Vβ11+ cells by I-E may begin at the transitional stage from Vβ11(low)CD4+CD8+ to Vβ11(high)CD4+CD8- single-position cells, and Vβ11(low)CD4+CD8+ cells differentiating to Vβ11(high)CD4-CD8+ cells seem to be resistant to clonal deletion. Vβ11+ T cells are also stimulated by staphylococcal enterotoxin A (SEA) irrespective of expression of CD4 or CD8. Nearly all of both Vβ11+CD4+ and Vβ11+CD8+ lymph node T cells were deleted by the injection of SEA every other day from birth. In their thymi, both Vβ11+CD4+CD8- and Vβ11+CD4-CD8+ single-positive thymocytes were deleted, and the proportion of Vβ11(low) thymocytes was lower than that of normal mice. The clonal deletion of Vβ11+ T cells by SEA injection occurs at a stage of immature Vβ11(low)CD4+CD8+ double-positive thymocytes, resulting in deletion of both Vβ11+CD4+ and Vβ11+CD8+ T cells.",
    author = "T. Ando and Y. Yoshikai and G. Matsuzaki and H. Takimoto and K. Nomoto",
    year = "1991",
    language = "English",
    volume = "74",
    pages = "638--644",
    journal = "Immunology",
    issn = "0019-2805",
    publisher = "Wiley-Blackwell",
    number = "4",

    }

    TY - JOUR

    T1 - The stage of negative selection in tolerance induction in neonatal mice

    AU - Ando, T.

    AU - Yoshikai, Y.

    AU - Matsuzaki, G.

    AU - Takimoto, H.

    AU - Nomoto, K.

    PY - 1991

    Y1 - 1991

    N2 - In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mls(b/a)) lymphoid cells, both CD4+ and CD8+ cells showed a striking reduction in the number of Vβ6+ cells capable of recognizing Mlsa in the context of major histocompatibility complex (MHC) class II molecules, indicating that clonal deletion of Vβ6+ cells by Mlsa antigen occurs just at a stage of immature Vβ6(low)CD4+CD8+ thymocytes. On the other hand, the number of Vβ11+ cells capable of recognizing I-E was markedly reduced in CD4+ cells, but CD8+ cells showed only partial (20%) reduction of such a population. The clonal deletion of Vβ11+ cells by I-E may begin at the transitional stage from Vβ11(low)CD4+CD8+ to Vβ11(high)CD4+CD8- single-position cells, and Vβ11(low)CD4+CD8+ cells differentiating to Vβ11(high)CD4-CD8+ cells seem to be resistant to clonal deletion. Vβ11+ T cells are also stimulated by staphylococcal enterotoxin A (SEA) irrespective of expression of CD4 or CD8. Nearly all of both Vβ11+CD4+ and Vβ11+CD8+ lymph node T cells were deleted by the injection of SEA every other day from birth. In their thymi, both Vβ11+CD4+CD8- and Vβ11+CD4-CD8+ single-positive thymocytes were deleted, and the proportion of Vβ11(low) thymocytes was lower than that of normal mice. The clonal deletion of Vβ11+ T cells by SEA injection occurs at a stage of immature Vβ11(low)CD4+CD8+ double-positive thymocytes, resulting in deletion of both Vβ11+CD4+ and Vβ11+CD8+ T cells.

    AB - In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mls(b/a)) lymphoid cells, both CD4+ and CD8+ cells showed a striking reduction in the number of Vβ6+ cells capable of recognizing Mlsa in the context of major histocompatibility complex (MHC) class II molecules, indicating that clonal deletion of Vβ6+ cells by Mlsa antigen occurs just at a stage of immature Vβ6(low)CD4+CD8+ thymocytes. On the other hand, the number of Vβ11+ cells capable of recognizing I-E was markedly reduced in CD4+ cells, but CD8+ cells showed only partial (20%) reduction of such a population. The clonal deletion of Vβ11+ cells by I-E may begin at the transitional stage from Vβ11(low)CD4+CD8+ to Vβ11(high)CD4+CD8- single-position cells, and Vβ11(low)CD4+CD8+ cells differentiating to Vβ11(high)CD4-CD8+ cells seem to be resistant to clonal deletion. Vβ11+ T cells are also stimulated by staphylococcal enterotoxin A (SEA) irrespective of expression of CD4 or CD8. Nearly all of both Vβ11+CD4+ and Vβ11+CD8+ lymph node T cells were deleted by the injection of SEA every other day from birth. In their thymi, both Vβ11+CD4+CD8- and Vβ11+CD4-CD8+ single-positive thymocytes were deleted, and the proportion of Vβ11(low) thymocytes was lower than that of normal mice. The clonal deletion of Vβ11+ T cells by SEA injection occurs at a stage of immature Vβ11(low)CD4+CD8+ double-positive thymocytes, resulting in deletion of both Vβ11+CD4+ and Vβ11+CD8+ T cells.

    UR - http://www.scopus.com/inward/record.url?scp=0026323330&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026323330&partnerID=8YFLogxK

    M3 - Article

    VL - 74

    SP - 638

    EP - 644

    JO - Immunology

    JF - Immunology

    SN - 0019-2805

    IS - 4

    ER -