The Ste16 WD-repeat protein regulates cell cycle progression under starvation through the Rum1 protein in Schizosaccharomyces pombe

Hiromi Maekawa, Kenji Kitamura, Chikashi Shimoda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The haploid cells of the fission yeast, Schizosaccharomyces pombe, are arrested in the G1-phase by nitrogen starvation and are committed to sexual reproduction (mating and sporulation). We isolated the sterile mutants which were defective in G1 arrest following nitrogen starvation. Genetic analysis of these mutants defined a single locus designated as ste16. The nucleotide sequence revealed that ste16+ encodes an 82-kDa protein containing eight WD40-repeats in its carboxy terminal half. The ste16 disruptant was viable, but arrested the cell cycle in the G2-phase after the nutritional down-shift. When transferred to fresh growth medium, the G2-arrested ste16Δ haploids resumed the mitotic cycle from the S-phase, resulting in diploidization. This diploidization phenomenon was completely suppressed by the null mutation of rum1 encoding the inhibitor of Cdc2 kinase. As the Rum1 protein level was remarkably elevated in the ste16Δ, the Ste16 protein negatively controls the Rum1 level. The loss of function of ste16 disturbs the cell-cycle progression and impairs the mechanism for the maintenance of ploidy.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalCurrent Genetics
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Schizosaccharomyces pombe Proteins
Cell Cycle Proteins
Starvation
Schizosaccharomyces
Haploidy
Cell Cycle
Nitrogen
Proteins
Ploidies
G2 Phase
G1 Phase
S Phase
Reproduction
Phosphotransferases
Maintenance
Mutation
Growth
WD40 Repeats

All Science Journal Classification (ASJC) codes

  • Genetics

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The Ste16 WD-repeat protein regulates cell cycle progression under starvation through the Rum1 protein in Schizosaccharomyces pombe. / Maekawa, Hiromi; Kitamura, Kenji; Shimoda, Chikashi.

In: Current Genetics, Vol. 33, No. 1, 01.01.1998, p. 29-37.

Research output: Contribution to journalArticle

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AB - The haploid cells of the fission yeast, Schizosaccharomyces pombe, are arrested in the G1-phase by nitrogen starvation and are committed to sexual reproduction (mating and sporulation). We isolated the sterile mutants which were defective in G1 arrest following nitrogen starvation. Genetic analysis of these mutants defined a single locus designated as ste16. The nucleotide sequence revealed that ste16+ encodes an 82-kDa protein containing eight WD40-repeats in its carboxy terminal half. The ste16 disruptant was viable, but arrested the cell cycle in the G2-phase after the nutritional down-shift. When transferred to fresh growth medium, the G2-arrested ste16Δ haploids resumed the mitotic cycle from the S-phase, resulting in diploidization. This diploidization phenomenon was completely suppressed by the null mutation of rum1 encoding the inhibitor of Cdc2 kinase. As the Rum1 protein level was remarkably elevated in the ste16Δ, the Ste16 protein negatively controls the Rum1 level. The loss of function of ste16 disturbs the cell-cycle progression and impairs the mechanism for the maintenance of ploidy.

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