The Structure and Characterization of the Surface Protein Layer of a Comamonas-Like Organism

Sun Nyunt Wai, Akemi Takade, Shuji Fujimoto, Kazunobu Amako

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The regular surface layer of a strain of a Comamonas-like organism was examined by electron microscopy. The surface layer protein was easily extracted from the cell surface by a 2.5 m solution of lithium chloride. The protein subunit has a molecular size of 32,000 daltons, but usually forms a large aggregate of more than 1,200,000 daltons. In the extract it formed a regular array of p4 symmetry and was observed to be intimately associated with fragments of lipopolysaccharide. The size of a subunit determined by the negative staining method and the image processing method measured 5.2 × 6.4 nm (width and length), was arranged in a cobblestone-like pattern, and was located in a lattice space measuring 13.0 nm square.

Original languageEnglish
Pages (from-to)943-949
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume39
Issue number12
DOIs
Publication statusPublished - Jan 1 1995

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Comamonas
Lithium Chloride
Negative Staining
Protein Subunits
Lipopolysaccharides
Electron Microscopy
S-layer proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

The Structure and Characterization of the Surface Protein Layer of a Comamonas-Like Organism. / Wai, Sun Nyunt; Takade, Akemi; Fujimoto, Shuji; Amako, Kazunobu.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 39, No. 12, 01.01.1995, p. 943-949.

Research output: Contribution to journalArticle

Wai, Sun Nyunt ; Takade, Akemi ; Fujimoto, Shuji ; Amako, Kazunobu. / The Structure and Characterization of the Surface Protein Layer of a Comamonas-Like Organism. In: MICROBIOLOGY and IMMUNOLOGY. 1995 ; Vol. 39, No. 12. pp. 943-949.
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