The structure of the archaebacterial ribosomal protein S7 and its possible interaction with 16S rRNA1

Harumi Hosaka, Min Yao, Makoto Kimura, Isao Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ribosomal protein S7 is one of the ubiquitous components of the small subunit of the ribosome. It is a 16S rRNA-binding protein positioned close to the exit of the tRNA, and it plays a role in initiating assembly of the head of the 30S subunit. Previous structural analyses of eubacterial S7 have shown that it has a stable α-helix core and a flexible β-arm. Unlike these eubacterial proteins, archaebacterial or eukaryotic S7 has an N-terminal extension of approximately 60 residues. The crystal structure of S7 from archaebacterium Pyrococcus horikoshii (PhoS7) has been determined at 2.1 Å resolution. The final model of PhoS7 consists of six major α-helices, a short 310-helix and two β-stands. The major part (residues 18-45) of the N-terminal extension of PhoS7 reinforces the α-helical core by well-extended hydrophobic interactions, while the other part (residues 46-63) is not visible in the crystal and is possibly fixed only by interacting with 16S rRNA. These differences in the N-terminal extension as well as in the insertion (between α1 and α2) of the archaebacterial S7 structure from eubacterial S7 are such that they do not necessitate a major change in the structure of the currently available eubacterial 16S rRNA. Some of the inserted chains might pass through gaps formed by helices of the 16S rRNA.

Original languageEnglish
Pages (from-to)695-701
Number of pages7
JournalJournal of biochemistry
Volume130
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

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ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Transfer RNA
Carrier Proteins
Crystal structure
Crystals
Proteins
Pyrococcus horikoshii
Small Ribosome Subunits
Archaea
Hydrophobic and Hydrophilic Interactions
Arm
Head
ribosomal protein S7

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

The structure of the archaebacterial ribosomal protein S7 and its possible interaction with 16S rRNA1. / Hosaka, Harumi; Yao, Min; Kimura, Makoto; Tanaka, Isao.

In: Journal of biochemistry, Vol. 130, No. 5, 01.01.2001, p. 695-701.

Research output: Contribution to journalArticle

Hosaka, Harumi ; Yao, Min ; Kimura, Makoto ; Tanaka, Isao. / The structure of the archaebacterial ribosomal protein S7 and its possible interaction with 16S rRNA1. In: Journal of biochemistry. 2001 ; Vol. 130, No. 5. pp. 695-701.
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