The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium

Norihiro Takekawa, Naoya Terahara, Takayuki Kato, Mizuki Gohara, Kouta Mayanagi, Atsushi Hijikata, Yasuhiro Onoue, Seiji Kojima, Tsuyoshi Shirai, Keiichi Namba, Michio Homma

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Rotation of bacterial flagellar motor is driven by the interaction between the stator and rotor, and the driving energy is supplied by ion influx through the stator channel. The stator is composed of the MotA and MotB proteins, which form a hetero-hexameric complex with a stoichiometry of four MotA and two MotB molecules. MotA and MotB are four-and single-transmembrane proteins, respectively. To generate torque, the MotA/MotB stator unit changes its conformation in response to the ion influx, and interacts with the rotor protein FliG. Here, we overproduced and purified MotA of the hyperthermophilic bacterium Aquifex aeolicus. A chemical crosslinking experiment revealed that MotA formed a multimeric complex, most likely a tetramer. The three-dimensional structure of the purified MotA, reconstructed by electron microscopy single particle imaging, consisted of a slightly elongated globular domain and a pair of arch-like domains with spiky projections, likely to correspond to the transmembrane and cytoplasmic domains, respectively. We show that MotA molecules can form a stable tetrameric complex without MotB, and for the first time, demonstrate the cytoplasmic structure of the stator.

    Original languageEnglish
    Article number31526
    JournalScientific reports
    Volume6
    DOIs
    Publication statusPublished - Aug 17 2016

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    Bacteria
    Cytoplasmic Structures
    Ions
    Proteins
    Torque
    Electron Microscopy

    All Science Journal Classification (ASJC) codes

    • General

    Cite this

    The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium. / Takekawa, Norihiro; Terahara, Naoya; Kato, Takayuki; Gohara, Mizuki; Mayanagi, Kouta; Hijikata, Atsushi; Onoue, Yasuhiro; Kojima, Seiji; Shirai, Tsuyoshi; Namba, Keiichi; Homma, Michio.

    In: Scientific reports, Vol. 6, 31526, 17.08.2016.

    Research output: Contribution to journalArticle

    Takekawa, N, Terahara, N, Kato, T, Gohara, M, Mayanagi, K, Hijikata, A, Onoue, Y, Kojima, S, Shirai, T, Namba, K & Homma, M 2016, 'The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium', Scientific reports, vol. 6, 31526. https://doi.org/10.1038/srep31526
    Takekawa, Norihiro ; Terahara, Naoya ; Kato, Takayuki ; Gohara, Mizuki ; Mayanagi, Kouta ; Hijikata, Atsushi ; Onoue, Yasuhiro ; Kojima, Seiji ; Shirai, Tsuyoshi ; Namba, Keiichi ; Homma, Michio. / The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium. In: Scientific reports. 2016 ; Vol. 6.
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    AU - Hijikata, Atsushi

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