The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome

Atsushi Nakagawa, Takashi Nakashima, Masae Taniguchi, Harumi Hosaka, Makoto Kimura, Isao Tanaka

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Ribosomal protein L2 is the largest protein component in the ribosome. It is located at or near the peptidyl transferase center and has been a prime candidate for the peptidyl transferase activity. It binds directly to 23S rRNA and plays a crucial role in its assembly. The three-dimensional structure of the RNA-binding domain of L2 from Bacillus stearothermophilus has been determined at 2.3 Å resolution by X-ray crystallography using the selenomethionyl MAD method. The RNA-binding domain of L2 consists of two recurring motifs of ~ 70 residues each. The N-terminal domain (positions 60-130) is homologous to the OB-fold, and the C-terminal domain (positions 131-201) is homologous to the SH3-like barrel. Residues Arg86 and Arg155, which have been identified by mutation experiments to be involved in the 23S rRNA binding, are located at the gate of the interface region between the two domains. The molecular architecture suggests how this important protein has evolved from the ancient nucleic acid-binding proteins to create a 23S rRNA-binding domain in the very remote past.

Original languageEnglish
Pages (from-to)1459-1467
Number of pages9
JournalEMBO Journal
Volume18
Issue number6
Publication statusPublished - Mar 15 1999

Fingerprint

Peptidyl Transferases
Ribosomes
RNA
Geobacillus stearothermophilus
X ray crystallography
X Ray Crystallography
Bacilli
Nucleic Acids
Carrier Proteins
Proteins
Mutation
Experiments
RNA-Binding Motifs
ribosomal protein L2
RNA Recognition Motif Proteins
mycophenolic adenine dinucleotide

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome. / Nakagawa, Atsushi; Nakashima, Takashi; Taniguchi, Masae; Hosaka, Harumi; Kimura, Makoto; Tanaka, Isao.

In: EMBO Journal, Vol. 18, No. 6, 15.03.1999, p. 1459-1467.

Research output: Contribution to journalArticle

Nakagawa, Atsushi ; Nakashima, Takashi ; Taniguchi, Masae ; Hosaka, Harumi ; Kimura, Makoto ; Tanaka, Isao. / The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome. In: EMBO Journal. 1999 ; Vol. 18, No. 6. pp. 1459-1467.
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