The Tol1 transposable element of the medaka fish moves in human and mouse cells

Akihiko Koga, Atsuko Shimada, Toshiya Kuroki, Hiroshi Hori, Junko Kusumi, Yoriko Kyono-Hamaguchi, Satoshi Hamaguchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

DNA-based transposable elements can be used as tools for gene engineering and gene therapy. A great advantage over RNA-mediated elements and retroviruses is the simplicity and safety of usage. The Tol1 element of the medaka fish Oryzias latipes has structural features of DNA-based elements. Although its excision has already been demonstrated, de novo insertion has not been observed, and a transposase has not been hitherto identified. We first cloned, through in silico search alignments and genomic library screenings, a 4.4-kb Tol1 copy carrying open reading frames and then identified, by mRNA analysis, a 2.9-kb transcript coding for 851 amino acids. The protein product of this transcript catalyzed transposition of a nonautonomous Tol1 copy in human and mouse culture cells. This identification of a fully functional Tol1 transposase could lead to the development of new tools for basic and translational molecular biology applications in mammals.

Original languageEnglish
Pages (from-to)628-635
Number of pages8
JournalJournal of Human Genetics
Volume52
Issue number7
DOIs
Publication statusPublished - Jun 1 2007

Fingerprint

Oryzias
Transposases
DNA Transposable Elements
Fishes
Genomic Library
Retroviridae
Genetic Therapy
Computer Simulation
Open Reading Frames
Molecular Biology
Mammals
Cell Culture Techniques
RNA
Safety
Amino Acids
Messenger RNA
DNA
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Koga, A., Shimada, A., Kuroki, T., Hori, H., Kusumi, J., Kyono-Hamaguchi, Y., & Hamaguchi, S. (2007). The Tol1 transposable element of the medaka fish moves in human and mouse cells. Journal of Human Genetics, 52(7), 628-635. https://doi.org/10.1007/s10038-007-0161-2

The Tol1 transposable element of the medaka fish moves in human and mouse cells. / Koga, Akihiko; Shimada, Atsuko; Kuroki, Toshiya; Hori, Hiroshi; Kusumi, Junko; Kyono-Hamaguchi, Yoriko; Hamaguchi, Satoshi.

In: Journal of Human Genetics, Vol. 52, No. 7, 01.06.2007, p. 628-635.

Research output: Contribution to journalArticle

Koga, A, Shimada, A, Kuroki, T, Hori, H, Kusumi, J, Kyono-Hamaguchi, Y & Hamaguchi, S 2007, 'The Tol1 transposable element of the medaka fish moves in human and mouse cells', Journal of Human Genetics, vol. 52, no. 7, pp. 628-635. https://doi.org/10.1007/s10038-007-0161-2
Koga, Akihiko ; Shimada, Atsuko ; Kuroki, Toshiya ; Hori, Hiroshi ; Kusumi, Junko ; Kyono-Hamaguchi, Yoriko ; Hamaguchi, Satoshi. / The Tol1 transposable element of the medaka fish moves in human and mouse cells. In: Journal of Human Genetics. 2007 ; Vol. 52, No. 7. pp. 628-635.
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