The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units

Junya Ishikawa; Nana Isomoto; Yuki Fujita; Hiroyuki Furuta; Yoshiya Ikawa

doi:10.1271/bbb.90849

The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units

Junya Ishikawa, Nana Isomoto, Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.

Original language	English
Pages (from-to)	872-874
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	74
Issue number	4
DOIs	https://doi.org/10.1271/bbb.90849
Publication status	Published - 2010

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.90849

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abstract = "TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.",

author = "Junya Ishikawa and Nana Isomoto and Yuki Fujita and Hiroyuki Furuta and Yoshiya Ikawa",

note = "Funding Information: This research was partly supported by Grants-in-Aid for Scientific Research from MEXT, Japan (no. 21111518 and no. 19657071 to Y.I., and the G-COE program {\textquoteleft}{\textquoteleft}Science for Future Molecular Systems{\textquoteright}{\textquoteright} to H.F.).",

year = "2010",

doi = "10.1271/bbb.90849",

language = "English",

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journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units

AU - Ishikawa, Junya

AU - Isomoto, Nana

AU - Fujita, Yuki

AU - Furuta, Hiroyuki

AU - Ikawa, Yoshiya

N1 - Funding Information: This research was partly supported by Grants-in-Aid for Scientific Research from MEXT, Japan (no. 21111518 and no. 19657071 to Y.I., and the G-COE program ‘‘Science for Future Molecular Systems’’ to H.F.).

PY - 2010

Y1 - 2010

N2 - TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.

AB - TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.

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DO - 10.1271/bbb.90849

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 4

ER -

The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units

Abstract

All Science Journal Classification (ASJC) codes

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