The transmembrane region of guard cell SLAC1 channels perceives CO2 signals via an ABA-independent pathway in arabidopsis

Yoshiko Yamamoto, Juntaro Negi, Cun Wang, Yasuhiro Isogai, Julian I. Schroeder, Koh Iba

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The guard cell S-type anion channel, SLOW ANION CHANNEL1 (SLAC1), a key component in the control of stomatal movements, is activated in response to CO2 and abscisic acid (ABA). Several amino acids existing in the N-terminal region of SLAC1 are involved in regulating its activity via phosphorylation in the ABA response. However, little is known about sites involved in CO2signal perception. To dissect sites that are necessary for the stomatal CO2 response, we performed slac1 complementation experiments using transgenic plants expressing truncated SLAC1 proteins. Measurements of gas exchange and stomatal apertures in the truncated transgenic lines in response to CO2 and ABA revealed that sites involved in the stomatal CO2 response exist in the transmembrane region and do not require the SLAC1 N and C termini. CO2 and ABA regulation of S-type anion channel activity in guard cells of the transgenic lines confirmed these results. In vivo sitedirected mutagenesis experiments targeted to amino acids within the transmembrane region of SLAC1 raise the possibility that two tyrosine residues exposed on the membrane are involved in the stomatal CO2 response.

Original languageEnglish
Pages (from-to)557-567
Number of pages11
JournalPlant Cell
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 2015

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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