The Two-synergistic Peptide Bacteriocin Produced by Enterococcus faecium NKR-5-3 Isolated from Thai Fermented Fish (Pla-ra)

Pongtep Wilaipun, Takeshi Zendo, Mathana Sangjindavong, Sunee Nitisinprasert, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Bacteriocin produced by Enterococcus faecium NKR-5-3 isolated from Thai fermented fish was characterized and purified. According to its physical and chemical properties, E. faecium NKR-5-3 produced heat tolerant bacteriocin with broad spectrum activity against indicator strains. Treatment of bacteriocin in cell-free neutralized supernatant (CFNS) with some proteases eliminated or reduced its activity, suggesting it is proteinaceous. Furthermore, it was stable in 0 to 24% NaCl, heat tolerant at pH 2 to 10 and bactericidal toward Enterococcus faecalis ATCC 19433T. Two antibacterial peptides, named enterocin NKR-5-3A and enterocin NKR-5-3B, were purified from the culture supernatant by four steps of column chromatography. Enterocin NKR-5-3A had the same mass, MW=5,241, and N-terminal amino acid sequence as brochocin A produced by Brochothrix campestris ATCC 43754, suggesting it may be identical to brochocin A. Enterocin NKR-5-3B exhibited MW=6,320, but its N-terminal amino acid sequence could not be determined. When these two purified peptides were mixed in a 1:1 ratio, the bacteriocin activity reached a maximum, which corresponded to 64 times the total calculated bacteriocin activity in the mixture, suggesting their synergism.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalScienceAsia
Volume30
Issue number2
DOIs
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • General

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