The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice

Jihwan Song, Suk P. Oh, Heinrich Schrewe, Masatoshi Nomura, Hong Lei, Makiko Okano, Thomas Gridley, En Li

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

The type II activin receptors, ActRIIA and ActRIIB, have been shown to play critical roles in axial patterning and organ development in mice. To investigate whether their function is required for mesoderm formation and gastrulation as implicated in Xenopus studies, we generated mice carrying both receptor mutations by interbreeding the ActRIIA and ActRIIB knockout mutants. We found that embryos homozygous for both receptor mutations were growth arrested at the egg cylinder stage and did not form mesoderm. Further analyses revealed that ActRIIA(-/-) ActRIIB(+/-) and about 15% of the ActRIIA(-/-) embryos failed to form an elongated primitive streak, resulting in severe disruption of mesoderm formation in the embryo proper. Interestingly, we observed similar gastrulation defects in ActRIIA(-/- )nodal(+/-) double mutants, which, if they developed beyond the gastrulation stage, displayed rostral head defects and cyclopia. These results provide genetic evidence that type II activin receptors are required for egg cylinder growth, primitive streak formation, and rotral head development in mice.

Original languageEnglish
Pages (from-to)157-169
Number of pages13
JournalDevelopmental Biology
Volume213
Issue number1
DOIs
Publication statusPublished - Sep 1 1999

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Type II Activin Receptors
Gastrulation
Mesoderm
Primitive Streak
Ovum
Embryonic Structures
Head
Growth
Mutation
Xenopus

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice. / Song, Jihwan; Oh, Suk P.; Schrewe, Heinrich; Nomura, Masatoshi; Lei, Hong; Okano, Makiko; Gridley, Thomas; Li, En.

In: Developmental Biology, Vol. 213, No. 1, 01.09.1999, p. 157-169.

Research output: Contribution to journalArticle

Song, J, Oh, SP, Schrewe, H, Nomura, M, Lei, H, Okano, M, Gridley, T & Li, E 1999, 'The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice', Developmental Biology, vol. 213, no. 1, pp. 157-169. https://doi.org/10.1006/dbio.1999.9370
Song, Jihwan ; Oh, Suk P. ; Schrewe, Heinrich ; Nomura, Masatoshi ; Lei, Hong ; Okano, Makiko ; Gridley, Thomas ; Li, En. / The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice. In: Developmental Biology. 1999 ; Vol. 213, No. 1. pp. 157-169.
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