The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation

Hiroki Sugimoto, Kensuke Kusumi, Yuzuru Tozawa, Junshi Yazaki, Naoki Kishimoto, Shoshi Kikuchi, Koh Iba

Research output: Contribution to journalArticle

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Abstract

The rice virescent-2 mutant (v2) is temperature conditional and develops chlorotic, chloroplast-deficient leaves at the restrictive temperature. In the v2 mutant, plastid-encoded proteins involved in photosynthesis and plastid transcriptional regulation were not detectable at any time during chloroplast differentiation. However, the plastid transcripts for these two classes of proteins behaved differently in the mutant, with those for the plastid transcription/ translation apparatus accumulating to wild-type levels and those for photosynthetic apparatus being suppressed. Polysome analysis showed that translation of the plastid transcripts encoding the plastid transcription/translation apparatus was blocked at an early stage of chloroplast differentiation. Accumulation of transcripts of nuclear-encoded photosynthetic genes, such as cab and rbcS, was strongly suppressed in the mutant at later stages of chloroplast differentiation, whereas transcripts of genes for the plastid transcription apparatus, such as OsRpoTp and OsSIG2A, accumulated to abnormally high levels at these stages. These results suggest that activation of the plastid translation machinery at an early stage of chloroplast differentiation is important for triggering the transmission of information about plastid developmental state to the nucleus, which in turn is required for the induction of nuclear-encoded chloroplast proteins at later stages of chloroplast differentiation.

Original languageEnglish
Pages (from-to)985-996
Number of pages12
JournalPlant and Cell Physiology
Volume45
Issue number8
DOIs
Publication statusPublished - Jan 1 2004

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Plastids
Chloroplasts
translation (genetics)
plastids
chloroplasts
mutation
Mutation
mutants
Chloroplast Proteins
transcription (genetics)
Temperature
Polyribosomes
Photosynthesis
polyribosomes
proteins
Genes
temperature
genes
photosynthesis
rice

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. / Sugimoto, Hiroki; Kusumi, Kensuke; Tozawa, Yuzuru; Yazaki, Junshi; Kishimoto, Naoki; Kikuchi, Shoshi; Iba, Koh.

In: Plant and Cell Physiology, Vol. 45, No. 8, 01.01.2004, p. 985-996.

Research output: Contribution to journalArticle

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