The volume-activated chloride current in human endothelial cells depends on intracellular ATP

Masahiro Oike, Guy Droogmans, Bernd Nilius

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have studied the effect of intracellular ATP on volume-activated Cl--currents in endothelial cells from human umbilical veins by means of the whole-cell patch clamp technique. The run-down of this current in ruptured patches during repetitive applications of hypotonic solutions (HTS) could be significantly reduced if the cells were internally perfused with a pipette solution that contained 4 mmol/l ATP. This run-down was much less pronounced if currents were recorded using nystatin-perforated patches. The amplitude of the current was drastically reduced and its activation became slower if the cells were superfused with a glucose-free medium with 1 mmol/l KCN. Adding 4 mmol/l ATPγS, a poorly hydrolyzable ATP-analogue, to the patch pipette prevented run-down of the current during repetitive activations by HTS, even if the cells were superfused with glucose-free solution with 1 mmol/l KCN. It is concluded that activation of the mechanosensitive Cl- conductance in human endothelial cells requires the presence of intracellular ATP, but not its hydrolysis.

Original languageEnglish
Pages (from-to)184-186
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume427
Issue number1-2
DOIs
Publication statusPublished - May 1 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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