Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition

Yi Ni, Chenchen Liu, Qinmiao Chen, Xifang Zhu, Xiaoming Dou

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Programmed step electric field strength is a simple-to-use technique that has already been reported to be effective to enhance the efficiency or speed of DNA electrophoresis. However, a global understanding and the details of this technique are still vague. In this paper, we investigated the influence of programmed step electric field strength by theoretical calculation and concentrated on a basic format named as two-step electric field strength. Both subtypes of two-step electric field strength conditions were considered. The important parameters, such as peak spacing, peak width, resolution, and migration time, were calculated in theory to understand the performance of DNA electrophoresis under programmed step electric field strength. The influence of two-step electric field strength on DNA electrophoresis was clearly revealed on a diagram of resolution versus migration time. Both resolution and speed of DNA electrophoresis under two-step electric field strength conditions are simply expressed by the shape of curves in the diagram. The possible shapes of curve were explored by calculation and shown in this paper. The subtype II of two-step electric field strength brings drastic variation on the resolution. Its limitations of enhancement and deterioration of resolution were predicted in theory.

Original languageEnglish
Pages (from-to)3638-3644
Number of pages7
JournalJournal of Separation Science
Volume38
Issue number20
DOIs
Publication statusPublished - Oct 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Fingerprint

Dive into the research topics of 'Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition'. Together they form a unique fingerprint.

Cite this