Abstract
Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.
| Original language | English |
|---|---|
| Article number | e90681 |
| Journal | PloS one |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 20 2014 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Cite this
Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa. / Kondo, Ryosuke; Ikiru, Atsuta; Ayukawa, Yasunori; Yamaza, Takayoshi; Matsuura, Yuri; Furuhashi, Akihiro; Tsukiyama, Yoshihiro; Koyano, Kiyoshi.
In: PloS one, Vol. 9, No. 3, e90681, 20.03.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa
AU - Kondo, Ryosuke
AU - Ikiru, Atsuta
AU - Ayukawa, Yasunori
AU - Yamaza, Takayoshi
AU - Matsuura, Yuri
AU - Furuhashi, Akihiro
AU - Tsukiyama, Yoshihiro
AU - Koyano, Kiyoshi
PY - 2014/3/20
Y1 - 2014/3/20
N2 - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.
AB - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.
UR - http://www.scopus.com/inward/record.url?scp=84925283675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925283675&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0090681
DO - 10.1371/journal.pone.0090681
M3 - Article
VL - 9
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e90681
ER -