Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

Ryosuke Kondo; Ikiru Atsuta; Yasunori Ayukawa; Takayoshi Yamaza; Yuri Matsuura; Akihiro Furuhashi; Yoshihiro Tsukiyama; Kiyoshi Koyano

doi:10.1371/journal.pone.0090681

Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

Ryosuke Kondo, Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Yuri Matsuura, Akihiro Furuhashi, Yoshihiro Tsukiyama, Kiyoshi Koyano

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

Original language	English
Article number	e90681
Journal	PloS one
Volume	9
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0090681
Publication status	Published - Mar 20 2014

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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10.1371/journal.pone.0090681

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title = "Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa",

abstract = "Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.",

author = "Ryosuke Kondo and Ikiru Atsuta and Yasunori Ayukawa and Takayoshi Yamaza and Yuri Matsuura and Akihiro Furuhashi and Yoshihiro Tsukiyama and Kiyoshi Koyano",

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AU - Kondo, Ryosuke

AU - Atsuta, Ikiru

AU - Ayukawa, Yasunori

AU - Yamaza, Takayoshi

AU - Matsuura, Yuri

AU - Furuhashi, Akihiro

AU - Tsukiyama, Yoshihiro

AU - Koyano, Kiyoshi

PY - 2014/3/20

Y1 - 2014/3/20

N2 - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

AB - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

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Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

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