Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

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Abstract

Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

Original languageEnglish
Article numbere90681
JournalPloS one
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 20 2014

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prostheses
Stem cells
Mesenchymal Stromal Cells
Dental Implants
mucosa
stem cells
Dental prostheses
Mucous Membrane
therapeutics
Rats
rats
Epithelium
Mesenchymal Stem Cell Transplantation
Bone
Therapeutics
epithelium
digestive tract mucosa
Mouth Mucosa
cell transplantation
Titanium

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa",
abstract = "Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.",
author = "Ryosuke Kondo and Atsuta Ikiru and Yasunori Ayukawa and Takayoshi Yamaza and Yuri Matsuura and Akihiro Furuhashi and Yoshihiro Tsukiyama and Kiyoshi Koyano",
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T1 - Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

AU - Kondo, Ryosuke

AU - Ikiru, Atsuta

AU - Ayukawa, Yasunori

AU - Yamaza, Takayoshi

AU - Matsuura, Yuri

AU - Furuhashi, Akihiro

AU - Tsukiyama, Yoshihiro

AU - Koyano, Kiyoshi

PY - 2014/3/20

Y1 - 2014/3/20

N2 - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

AB - Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants. Materials and Methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats. Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post- transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells. Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

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