Thermal separation: Interplay between the soret effect and entropic force gradient

Yusuke Maeda, Axel Buguin, Albert Libchaber

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Thermophoresis, the Soret effect, depletes a high concentration of a polyethylene glycol polymer solution from the hot region and builds a concentration gradient. In such a solution, solutes of small concentration experience thermophoresis and polyethylene glycol concentration-dependent restoring forces. We report that by using focused laser heating and varying the polyethylene glycol concentration one observes geometrical localizations of solutes like DNA and RNA into patterns such as a ring. For DNA up to 5.6 kbp, the ring size decreases following a behavior analogous to a gel electrophoresis separation. Above 5.6 kbp, the ring diameter increases with the DNA length. Mixtures of DNA and RNA can be separated as well as different RNA lengths. Separation of colloids is also observed. The experiments might be relevant for the separation of small RNA ribozymes in an early stage of life.

Original languageEnglish
Article number038301
JournalPhysical Review Letters
Volume107
Issue number3
DOIs
Publication statusPublished - Jul 11 2011

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gradients
deoxyribonucleic acid
thermophoresis
glycols
polyethylenes
rings
solutes
laser heating
electrophoresis
colloids
gels
polymers

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Thermal separation : Interplay between the soret effect and entropic force gradient. / Maeda, Yusuke; Buguin, Axel; Libchaber, Albert.

In: Physical Review Letters, Vol. 107, No. 3, 038301, 11.07.2011.

Research output: Contribution to journalArticle

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