Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)

Ning Chen, Anatoly A. Zinchenko, Satoru Kidoaki, Shizuaki Murata, Kenichi Yoshikawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. "With this method, the conformation of long genomic DNA can be readily and reversibly switched, between a very compact condensate and an unfolded macromolecule.

Original languageEnglish
Pages (from-to)2995-2998
Number of pages4
JournalLangmuir
Volume26
Issue number5
DOIs
Publication statusPublished - Mar 2 2010

Fingerprint

Conformations
DNA
deoxyribonucleic acid
Macromolecules
macromolecules
condensates
Molecules
poly-N-isopropylacrylamide
molecules
Temperature
temperature

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide). / Chen, Ning; Zinchenko, Anatoly A.; Kidoaki, Satoru; Murata, Shizuaki; Yoshikawa, Kenichi.

In: Langmuir, Vol. 26, No. 5, 02.03.2010, p. 2995-2998.

Research output: Contribution to journalArticle

Chen, Ning ; Zinchenko, Anatoly A. ; Kidoaki, Satoru ; Murata, Shizuaki ; Yoshikawa, Kenichi. / Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide). In: Langmuir. 2010 ; Vol. 26, No. 5. pp. 2995-2998.
@article{d37834e20ba54377b485404002d7b2c1,
title = "Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)",
abstract = "We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. {"}With this method, the conformation of long genomic DNA can be readily and reversibly switched, between a very compact condensate and an unfolded macromolecule.",
author = "Ning Chen and Zinchenko, {Anatoly A.} and Satoru Kidoaki and Shizuaki Murata and Kenichi Yoshikawa",
year = "2010",
month = "3",
day = "2",
doi = "10.1021/la904375k",
language = "English",
volume = "26",
pages = "2995--2998",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)

AU - Chen, Ning

AU - Zinchenko, Anatoly A.

AU - Kidoaki, Satoru

AU - Murata, Shizuaki

AU - Yoshikawa, Kenichi

PY - 2010/3/2

Y1 - 2010/3/2

N2 - We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. "With this method, the conformation of long genomic DNA can be readily and reversibly switched, between a very compact condensate and an unfolded macromolecule.

AB - We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. "With this method, the conformation of long genomic DNA can be readily and reversibly switched, between a very compact condensate and an unfolded macromolecule.

UR - http://www.scopus.com/inward/record.url?scp=77749333037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77749333037&partnerID=8YFLogxK

U2 - 10.1021/la904375k

DO - 10.1021/la904375k

M3 - Article

VL - 26

SP - 2995

EP - 2998

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 5

ER -