Thermophilic Enterococcus faecium QU 50 enabled open repeated batch fermentation for l-lactic acid production from mixed sugars without carbon catabolite repression

J. Tan, Mohamed Ali Sayed Mohamed Abdelrahman, M. Numaguchi, Yukihiro Tashiro, Takeshi Zendo, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Enterococcus faecium QU 50, a novel thermophilic l-lactic acid (LA) producing strain, was used in this study to ferment sugar mixtures into LA. Under the optimal fermentation conditions (50 °C, pH 6.5), strain QU 50 could ferment both mixed glucose/xylose sugars with relaxed CCR and mixed cellobiose/xylose sugars simultaneously without CCR to produce homo l-LA. The activity of enzymes related to xylose metabolism was also investigated. In the cells grown in a medium containing cellobiose/xylose, the activity of xylose isomerase and xylulose kinase, was 3.22 and 1.91 times higher, respectively, as compared to that of cells grown in a glucose/xylose medium. Strain QU 50 produced 70.8 g L-1 of l-LA with a yield of 1.04 g g-1 and a productivity of 2.95 g L-1 h-1 from simulated energy cane hydrolysate in batch fermentation. Immobilisation of strain QU 50 improved the operational stability of open repeated fermentation (three cycles), resulting in 61.1-64.3 g L-1 of l-LA with a yield of 1.01-1.02 g g-1 and a productivity of 3.22-3.82 g L-1 h-1. Thus, an efficient and cost-effective fermentation system was successfully established for l-LA production from sugar mixtures.

Original languageEnglish
Pages (from-to)24233-24241
Number of pages9
JournalRSC Advances
Volume7
Issue number39
DOIs
Publication statusPublished - Jan 1 2017

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Xylose
Lactic acid
Sugars
Fermentation
Lactic Acid
Carbon
Cellobiose
xylose isomerase
Glucose
Productivity
Cells
Metabolism
Enzymes
Costs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Thermophilic Enterococcus faecium QU 50 enabled open repeated batch fermentation for l-lactic acid production from mixed sugars without carbon catabolite repression. / Tan, J.; Abdelrahman, Mohamed Ali Sayed Mohamed; Numaguchi, M.; Tashiro, Yukihiro; Zendo, Takeshi; Sakai, Kenji; Sonomoto, Kenji.

In: RSC Advances, Vol. 7, No. 39, 01.01.2017, p. 24233-24241.

Research output: Contribution to journalArticle

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