Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs

Koji Mori, Atsushi Yamazoe, Akira Hosoyama, Shoko Ohji, Nobuyuki Fujita, Jun Ichiro Ishibashi, Hiroyuki Kimura, Ken Ichiro Suzuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06T and AZM44c09T, were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06T were 60 °C, pH 7.4 and 0% additional NaCl, and those for strain AZM44c09T were 70 °C, pH 7.4 and 0% additional NaCl. Complete genome sequencing was performed for both strains, revealing genome sizes of 2.19 Mbp (AZM34c06T) and 2.01 Mbp (AZM44c09T). Phylogenetic analyses based on 16S rRNA gene sequences and the concatenated predicted amino acid sequences of 33 ribosomal proteins showed that both strains belonged to the genus Thermotoga. The closest relatives of strains AZM34c06T and AZM44c09T were the type strains of Thermotoga lettingae (96.0% similarity based on the 16S rRNA gene and 84.1% similarity based on ribosomal proteins) and Thermotoga hypogea (98.6 and 92.7% similarity), respectively. Using BLAST, the average nucleotide identity was 70.4-70.5% when comparing strain AZM34c06T and T. lettingae TMOT and 76.6% when comparing strain AZM44c09T and T. hypogea NBRC 106472T. Both values are far below the 95% threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Thermotoga within two novel species, Thermotoga profunda sp. nov. (type strain AZM34c06T = NBRC 106115T = DSM 23275T) and Thermotoga caldifontissp. nov. (type strain AZM44c09T = NBRC 106116T = DSM 23272T).

Original languageEnglish
Article number060137
Pages (from-to)2128-2136
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 6
DOIs
Publication statusPublished - Jun 2014

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Thermotoga
Hot Springs
thermophilic bacterium
thermophilic bacteria
hot springs
Anaerobic Bacteria
Ribosomal Proteins
thermal spring
rRNA Genes
Gram-Negative Anaerobic Bacteria
genome
Genome Size
protein
gene
new species
Amino Acid Sequence
Japan
Nucleotides
amino acid
Genome

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs. / Mori, Koji; Yamazoe, Atsushi; Hosoyama, Akira; Ohji, Shoko; Fujita, Nobuyuki; Ishibashi, Jun Ichiro; Kimura, Hiroyuki; Suzuki, Ken Ichiro.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 6, 060137, 06.2014, p. 2128-2136.

Research output: Contribution to journalArticle

Mori, Koji ; Yamazoe, Atsushi ; Hosoyama, Akira ; Ohji, Shoko ; Fujita, Nobuyuki ; Ishibashi, Jun Ichiro ; Kimura, Hiroyuki ; Suzuki, Ken Ichiro. / Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. PART 6. pp. 2128-2136.
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abstract = "Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06T and AZM44c09T, were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06T were 60 °C, pH 7.4 and 0{\%} additional NaCl, and those for strain AZM44c09T were 70 °C, pH 7.4 and 0{\%} additional NaCl. Complete genome sequencing was performed for both strains, revealing genome sizes of 2.19 Mbp (AZM34c06T) and 2.01 Mbp (AZM44c09T). Phylogenetic analyses based on 16S rRNA gene sequences and the concatenated predicted amino acid sequences of 33 ribosomal proteins showed that both strains belonged to the genus Thermotoga. The closest relatives of strains AZM34c06T and AZM44c09T were the type strains of Thermotoga lettingae (96.0{\%} similarity based on the 16S rRNA gene and 84.1{\%} similarity based on ribosomal proteins) and Thermotoga hypogea (98.6 and 92.7{\%} similarity), respectively. Using BLAST, the average nucleotide identity was 70.4-70.5{\%} when comparing strain AZM34c06T and T. lettingae TMOT and 76.6{\%} when comparing strain AZM44c09T and T. hypogea NBRC 106472T. Both values are far below the 95{\%} threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Thermotoga within two novel species, Thermotoga profunda sp. nov. (type strain AZM34c06T = NBRC 106115T = DSM 23275T) and Thermotoga caldifontissp. nov. (type strain AZM44c09T = NBRC 106116T = DSM 23272T).",
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AU - Mori, Koji

AU - Yamazoe, Atsushi

AU - Hosoyama, Akira

AU - Ohji, Shoko

AU - Fujita, Nobuyuki

AU - Ishibashi, Jun Ichiro

AU - Kimura, Hiroyuki

AU - Suzuki, Ken Ichiro

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