Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost

A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04^T, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04^T showed 99.4 % similarity with Bacillus thermolactis R-6488^T, 94.1 % similarity with Bacillus thermoamylovorans CNCM I-1378^T, 93.3 % similarity with Bacillus humi LMG 22167^T, 93.2 % similarity with Bacillus niacini IFO 15566^T and the similarities with other species were less than 93 %. DNA-DNA relatedness between strain MO-04^Tand B. thermolactis DSM 23332^T was 45 %. The DNA G+C content of strain MO-04^T was 33.4 mol%, comparatively lower than that of B. thermolactis R-6488^T (35.0 mol%). Strain MO-04^T grew at 35-61 °C (optimum 50 °C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04^T cell wall peptidoglycan type was meso-2, 6-diaminopimelic acid, and the major fatty acids were C_{16: 1}, C_{14: 1}, C_{17: 0} and C_{17: 1}. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04^T is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformissp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04^T (= JCM 19325^T = KCTC 33163^T).