Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost

Pramod Poudel, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Yukihiro Tashiro, Kenji Sakai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04T, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04T showed 99.4 % similarity with Bacillus thermolactis R-6488T, 94.1 % similarity with Bacillus thermoamylovorans CNCM I-1378T, 93.3 % similarity with Bacillus humi LMG 22167T, 93.2 % similarity with Bacillus niacini IFO 15566T and the similarities with other species were less than 93 %. DNA-DNA relatedness between strain MO-04Tand B. thermolactis DSM 23332T was 45 %. The DNA G+C content of strain MO-04T was 33.4 mol%, comparatively lower than that of B. thermolactis R-6488T (35.0 mol%). Strain MO-04T grew at 35-61 °C (optimum 50 °C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04T cell wall peptidoglycan type was meso-2, 6-diaminopimelic acid, and the major fatty acids were C16: 1, C14: 1, C17: 0 and C17: 1. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04T is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformissp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04T (= JCM 19325T = KCTC 33163T).

Original languageEnglish
Pages (from-to)2668-2674
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 8
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Bacillus (bacteria)
compost
Bacillus
composts
Soil
DNA
new species
animals
phospholipid
relatedness
Bacillus niacini
Gram-Positive Endospore-Forming Rods
Bacillus thermoamylovorans
fatty acid
lipid
Diaminopimelic Acid
Phosphatidylglycerols
endospores
bacterium
Cardiolipins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost. / Poudel, Pramod; Miyamoto, Hirokuni; Miyamoto, Hisashi; Okugawa, Yuki; Tashiro, Yukihiro; Sakai, Kenji.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 8, 01.01.2014, p. 2668-2674.

Research output: Contribution to journalArticle

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abstract = "A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04T, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04T showed 99.4 {\%} similarity with Bacillus thermolactis R-6488T, 94.1 {\%} similarity with Bacillus thermoamylovorans CNCM I-1378T, 93.3 {\%} similarity with Bacillus humi LMG 22167T, 93.2 {\%} similarity with Bacillus niacini IFO 15566T and the similarities with other species were less than 93 {\%}. DNA-DNA relatedness between strain MO-04Tand B. thermolactis DSM 23332T was 45 {\%}. The DNA G+C content of strain MO-04T was 33.4 mol{\%}, comparatively lower than that of B. thermolactis R-6488T (35.0 mol{\%}). Strain MO-04T grew at 35-61 °C (optimum 50 °C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0 {\%} (w/v) NaCl (optimum 2 {\%}). The MO-04T cell wall peptidoglycan type was meso-2, 6-diaminopimelic acid, and the major fatty acids were C16: 1, C14: 1, C17: 0 and C17: 1. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04T is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformissp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04T (= JCM 19325T = KCTC 33163T).",
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AU - Miyamoto, Hirokuni

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AU - Tashiro, Yukihiro

AU - Sakai, Kenji

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N2 - A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04T, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04T showed 99.4 % similarity with Bacillus thermolactis R-6488T, 94.1 % similarity with Bacillus thermoamylovorans CNCM I-1378T, 93.3 % similarity with Bacillus humi LMG 22167T, 93.2 % similarity with Bacillus niacini IFO 15566T and the similarities with other species were less than 93 %. DNA-DNA relatedness between strain MO-04Tand B. thermolactis DSM 23332T was 45 %. The DNA G+C content of strain MO-04T was 33.4 mol%, comparatively lower than that of B. thermolactis R-6488T (35.0 mol%). Strain MO-04T grew at 35-61 °C (optimum 50 °C), pH 4.5-9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04T cell wall peptidoglycan type was meso-2, 6-diaminopimelic acid, and the major fatty acids were C16: 1, C14: 1, C17: 0 and C17: 1. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04T is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformissp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04T (= JCM 19325T = KCTC 33163T).

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