Thermus thermophilus TMY isolated from silica scale taken from a geothermal power plant

Yasuhiro Fujino, R. Kawatsu, F. Inagaki, A. Umeda, T. Yokoyama, Yoshihiro Okaue, S. Iwai, S. Ogata, T. Ohshima, Katsumi Doi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aims: To identify an extreme thermophile, strain TMY, isolated from silica scale from the geothermal electric power plant and to examine microdiversity of Thermus thermophilus strains. Materials and Results: The isolated strain TMY was identified by morphological, biochemical and physiological tests. Phylogenetic comparison of the strain and other Thermus strains with 16S rDNA analysis, RAPD and ERIC-PCR fingerprinting were performed. Strain TMY was closely related to strain which was isolated from a hot spring in New Zealand and shown to belong to the Japanese Thermus cluster. However, there were considerable genetic differences between strain TMY and other Thermus species using DNA fingerprinting. Conclusions: Based on morphological, physiological and genetic properties, strain TMY could be a strain of T. thermophilus. The distinct properties of strain TMY suggest that microdiversity of T. thermophilus strains should be considered. Significance and Impact of the Study: The results of this study have demonstrated genetic diversity within T. thermophilus strains, which were previously masked by an almost identical 16S rDNA sequence. RAPD and ERIC-PCR could be potential methods for distinguishing between Thermus strains.

Original languageEnglish
Pages (from-to)70-78
Number of pages9
JournalJournal of Applied Microbiology
Volume104
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Thermus thermophilus
Thermus
Power Plants
power plants
Silicon Dioxide
silica
Random Amplified Polymorphic DNA Technique
Ribosomal DNA
Hot Springs
DNA Fingerprinting
New Zealand
thermophilic microorganisms
electric power
hot springs

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Thermus thermophilus TMY isolated from silica scale taken from a geothermal power plant. / Fujino, Yasuhiro; Kawatsu, R.; Inagaki, F.; Umeda, A.; Yokoyama, T.; Okaue, Yoshihiro; Iwai, S.; Ogata, S.; Ohshima, T.; Doi, Katsumi.

In: Journal of Applied Microbiology, Vol. 104, No. 1, 01.2008, p. 70-78.

Research output: Contribution to journalArticle

Fujino, Yasuhiro ; Kawatsu, R. ; Inagaki, F. ; Umeda, A. ; Yokoyama, T. ; Okaue, Yoshihiro ; Iwai, S. ; Ogata, S. ; Ohshima, T. ; Doi, Katsumi. / Thermus thermophilus TMY isolated from silica scale taken from a geothermal power plant. In: Journal of Applied Microbiology. 2008 ; Vol. 104, No. 1. pp. 70-78.
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