Thiazolidinedione (pioglitazone) blocks P. gingivalis- and F. nucleatum, but not E. coli, lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) production in adipocytes

M. Yamaguchi, Fusanori Nishimura, H. Naruishi, Y. Soga, S. Kokeguchi, S. Takashiba

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An elevated level of C-reactive protein (CRP) predicts the future development of coronary heart disease. Periodontitis appears to up-regulate CRP. CRP is produced by hepatocytes in response to interleukin-6 (IL-6). A major source of IL-6 in obese subjects is adipocytes. We hypothesized that lipopolysaccharide (LPS) from periodontal pathogens stimulated adipocytes to produce IL-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione (pioglitazone), since this agent potentially showed an anti-inflammatory effect. Mouse 3T3-L1 adipocytes were stimulated with E. coli, P. gingivalis, and F. nucleatum LPS. The IL-6 concentration in culture supernatants was measured. All LPS stimulated adipocytes to produce IL-6. Although pioglitazone changed adipocyte appearance from large to small, and completely suppressed P. gingivalis and F. nucleatum LPS-induced IL-6 production, E. coli LPS-induced IL-6 production was not efficiently blocked. Thus, pioglitazone completely blocked periodontal-bacteria- derived LPS-induced IL-6 production in adipocytes, a major inducer of CRP.

Original languageEnglish
Pages (from-to)240-244
Number of pages5
JournalJournal of Dental Research
Volume84
Issue number3
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

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pioglitazone
Adipocytes
Lipopolysaccharides
Interleukin-6
Escherichia coli
C-Reactive Protein
2,4-thiazolidinedione
Periodontitis

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Thiazolidinedione (pioglitazone) blocks P. gingivalis- and F. nucleatum, but not E. coli, lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) production in adipocytes. / Yamaguchi, M.; Nishimura, Fusanori; Naruishi, H.; Soga, Y.; Kokeguchi, S.; Takashiba, S.

In: Journal of Dental Research, Vol. 84, No. 3, 01.01.2005, p. 240-244.

Research output: Contribution to journalArticle

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