Thiobencarb herbicide reduces growth, photosynthetic activity, and amount of rieske iron-sulfur protein in the diatom Thalassiosira pseudonana

Yohei Shimasaki, Michito Tsuyama, Rumana Tasmin, Xuchun Qiu, Motoyuki Shimizu, Yusuke Sato, Yasuhiro Yamasaki, Yoko Kato-Unoki, Atsushi Nukata, Takashi Nakashima, Hirofumi Ichinose, Hiroyuki Wariishi, Tsuneo Honjo, Yuji Oshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity (Fv/Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb.

Original languageEnglish
Pages (from-to)437-444
Number of pages8
JournalJournal of Biochemical and Molecular Toxicology
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 1 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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