TY - JOUR
T1 - Three-dimensional atlas of thoracic ganglia in the praying mantis, Tenodera aridifolia
AU - Fujiki, Kentaro
AU - Nagase, Mihoko
AU - Takaki, Keigo
AU - Watanabe, Hidehiro
AU - Yamawaki, Yoshifumi
N1 - Funding Information:
The authors are grateful to Dr Toshio Ichikawa for his advices on the HE staining technique, to Dr Peter J Simmons for reading the manuscript and providing valuable comments, and Dr Tomoki Kazawa for his assistance in releasing our data on the CNS‐PF website. This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP17K07488 to Y.Y. The authors also thank Jeremy Allen, PhD, from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/6/15
Y1 - 2020/6/15
N2 - The praying mantis is a good model for the study of motor control, especially for investigating the transformation from sensory signals into motor commands. In insects, thoracic ganglia (TG) play an important role in motor control. To understand the functional organization of TG, an atlas is useful. However, except for the fruitfly, no three-dimensional atlas of TG has not been reported for insects. In this study, we generated a three-dimensional atlas of prothoracic, mesothoracic, and metathoracic ganglia in the praying mantis (Tenodera aridifolia). First, we observed serial sections of the prothoracic ganglion stained with hematoxylin and eosin to identify longitudinal tracts and transverse commissures. We then visualized neuropil areas by immunostaining whole-mount TG with an anti-synapsin antibody. Before labeling each neuropil area, standardization using the iterative shape averaging method was applied to images to make neuropil contours distinct. Neuropil areas in TG were defined based on their shape and relative position to tracts and commissures. Finally, a three-dimensional atlas was reconstructed from standardized images of the TG. The standard TG are available at the Comparative Neuroscience Platform website (cns.neuroinf.jp/modules/xoonips/detail.php?item_id=11946) and can be used as a common reference map to combine the anatomical data obtained from different individuals.
AB - The praying mantis is a good model for the study of motor control, especially for investigating the transformation from sensory signals into motor commands. In insects, thoracic ganglia (TG) play an important role in motor control. To understand the functional organization of TG, an atlas is useful. However, except for the fruitfly, no three-dimensional atlas of TG has not been reported for insects. In this study, we generated a three-dimensional atlas of prothoracic, mesothoracic, and metathoracic ganglia in the praying mantis (Tenodera aridifolia). First, we observed serial sections of the prothoracic ganglion stained with hematoxylin and eosin to identify longitudinal tracts and transverse commissures. We then visualized neuropil areas by immunostaining whole-mount TG with an anti-synapsin antibody. Before labeling each neuropil area, standardization using the iterative shape averaging method was applied to images to make neuropil contours distinct. Neuropil areas in TG were defined based on their shape and relative position to tracts and commissures. Finally, a three-dimensional atlas was reconstructed from standardized images of the TG. The standard TG are available at the Comparative Neuroscience Platform website (cns.neuroinf.jp/modules/xoonips/detail.php?item_id=11946) and can be used as a common reference map to combine the anatomical data obtained from different individuals.
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U2 - 10.1002/cne.24841
DO - 10.1002/cne.24841
M3 - Article
C2 - 31846077
AN - SCOPUS:85077362277
SN - 0021-9967
VL - 528
SP - 1599
EP - 1615
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 9
ER -