Abstract
Thispaper reports a novel method to concentrate bacteria in three-dimension by negative dielectrophoretic (n-DEP) force in a microchannel. This was achieved by placing a thin dielectric layer on one of a pair of parallel plate electrodes. The dielectric layer having a home-plate like pentagonal shape, forms a gradient of electric field causing n-DEP. A three-dimensional numerical simulation of bacteria trajectory predicts that bacteria flowing a microchannel were three-dimensionally concentrated beneath the tip of the pentagonal dielectric thin layer. The trajectory and concentration of bacteria under n-DEP force were also experimentally confirmed using Escherichia coli cells. Bacteria moved along edges of the dielectric layer and were pushed to the opposite electrode, resulting in their concentration in three-dimension. The proposed device might be applicable to selective concentration of bacteria depending on their dielectric properties.
| Original language | English |
|---|---|
| Title of host publication | Materials Science and Chemical Engineering |
| Pages | 251-256 |
| Number of pages | 6 |
| DOIs | |
| Publication status | Published - Jul 8 2013 |
| Event | 2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013 - , Singapore Duration: Feb 20 2013 → Feb 21 2013 |
Publication series
| Name | Advanced Materials Research |
|---|---|
| Volume | 699 |
| ISSN (Print) | 1022-6680 |
Other
| Other | 2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013 |
|---|---|
| Country | Singapore |
| Period | 2/20/13 → 2/21/13 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Engineering(all)
Cite this
Three dimensional bacteria concentration by negative dielectrophoresis. / Hisajima, T.; mao, L.; Shinzato, K.; Nakano, Michihiko; Suehiro, Junya.
Materials Science and Chemical Engineering. 2013. p. 251-256 (Advanced Materials Research; Vol. 699).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Three dimensional bacteria concentration by negative dielectrophoresis
AU - Hisajima, T.
AU - mao, L.
AU - Shinzato, K.
AU - Nakano, Michihiko
AU - Suehiro, Junya
PY - 2013/7/8
Y1 - 2013/7/8
N2 - Thispaper reports a novel method to concentrate bacteria in three-dimension by negative dielectrophoretic (n-DEP) force in a microchannel. This was achieved by placing a thin dielectric layer on one of a pair of parallel plate electrodes. The dielectric layer having a home-plate like pentagonal shape, forms a gradient of electric field causing n-DEP. A three-dimensional numerical simulation of bacteria trajectory predicts that bacteria flowing a microchannel were three-dimensionally concentrated beneath the tip of the pentagonal dielectric thin layer. The trajectory and concentration of bacteria under n-DEP force were also experimentally confirmed using Escherichia coli cells. Bacteria moved along edges of the dielectric layer and were pushed to the opposite electrode, resulting in their concentration in three-dimension. The proposed device might be applicable to selective concentration of bacteria depending on their dielectric properties.
AB - Thispaper reports a novel method to concentrate bacteria in three-dimension by negative dielectrophoretic (n-DEP) force in a microchannel. This was achieved by placing a thin dielectric layer on one of a pair of parallel plate electrodes. The dielectric layer having a home-plate like pentagonal shape, forms a gradient of electric field causing n-DEP. A three-dimensional numerical simulation of bacteria trajectory predicts that bacteria flowing a microchannel were three-dimensionally concentrated beneath the tip of the pentagonal dielectric thin layer. The trajectory and concentration of bacteria under n-DEP force were also experimentally confirmed using Escherichia coli cells. Bacteria moved along edges of the dielectric layer and were pushed to the opposite electrode, resulting in their concentration in three-dimension. The proposed device might be applicable to selective concentration of bacteria depending on their dielectric properties.
UR - http://www.scopus.com/inward/record.url?scp=84879627563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879627563&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.699.251
DO - 10.4028/www.scientific.net/AMR.699.251
M3 - Conference contribution
AN - SCOPUS:84879627563
SN - 9783037856758
T3 - Advanced Materials Research
SP - 251
EP - 256
BT - Materials Science and Chemical Engineering
ER -